Mouse Monoclonal Antibodies Isotyping Plates

Coated 96-Well Plates for Mouse Monoclonal Antibodies Isotyping enable easy and accurate identification of mouse immunoglobulin by ELISA Assay

An important step during the production of a monoclonal antibody is the knowledge of its isotype, since it allows to optimize the choice of the method of its purification. Isotyping helps the choice because determines the class (e.g. IgG vs IgA) and subclass(e.g. IgG2a vs IgG2b) of a monoclonal antibody...
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Biomat Mouse Monoclonal Antibody Isotyping plate enables easy identification of mouse immunoglobulin class, subclass and light chain. The microplate can be used performing an ELISA strip-well plates with individual wells precoated with goat anti-mouse Igs Fc specific capture antibody (anti IgG1, IgG2a, IgG2b, IgG3, IgA, IgM) and goat anti-mouse light chain (kappa and lambda) capture antibody. A mouse monoclonal antibody sample applied to the wells can be isotyped within two hours. Results can be evaluated qualitatively by visual inspection or quantitatively by measuring the absorbance at 450 nm (i.e. if using a goat anti-mouse HRP conjugate as detector).

Coated 96-Well Plates for Mab Isotyping main features

Biomat Coated Plates for Mouse Monoclonal Antibodies Isotyping are suitable with ELISA Assay Technology due to these features:

  • Ready to use
  • Manufactured under ISO:9001 guidelines
  • Made in pure polystyrene with low fluorescence
  • Coated with goat anti mouse heavy chain (IgG subclasses 1, 2a, 2b, 3 and IgA and IgM classes) and goat anti mouse light chain (kappa and lambda)
  • Eight well strip format allows a convenient partial use of the plate; use one strip (column) for each sample (12 samples per plate)
  • Characterize specific antibodies for six different subclasses and two different light-chain types
  • Accurate reactivity and specificity characterization with samples containing at least 0.05-1 µg/ml of IgG and 0.1-0.2 µg/ml of IgA and IgM
  • High compatibility using samples coming from hybridoma cell culture supernatant, ascites fluid, or purified antibodies
  • No special equipment is needed to process the microplate; assess results qualitatively evaluated visually or measured quantitatively using an ordinary ELISA plate reader
  • Post-coated (blocked) for low non specific binding and long-term stability
  • Alphanumeric coding for easy well recognition
  • The rim protects the external face of the bottom from scratches
  • Microplates comply with SBS standards and the design assures a good performance in automatic processing plant
  • All lots are tested for uniformity and reproducibility
  • Certificate of Quality is released for every lot
  • For Research Use Only