The Biomat Mouse Monoclonal Antibodies Isotyping Plates product is a 96 well microplate coated with goat anti mouse Igs Fc specific (IgG subclasses 1, 2a, 2b, 3 and IgA and IgM classes) and goat anti mouse light chain (kappa and lambda) for qualitative isotype determination of mouse immunoglobulins. This plate enables accurate identification of mouse immunoglobulin isotypes, including IgG1, IgG2a, IgG2b, IgG3, IgM and IgA from purified antibodies by capture Enzyme Linked Immunosorbent Assay (ELISA).
Isotyping involves determining the class (e.g. IgG vs IgA) and subclass (e.g. IgG2a vs IgG2b) of a monoclonal antibody: this is a critical step in antibody production as it is necessary for choosing an appropriate purification and modification method for the antibody.
Biomat Mouse Monoclonal Antibody Isotyping plate enables easy identification of mouse immunoglobulin class, subclass and light chain. The microplate can be used performing an ELISA strip-well plates with individual wells precoated with goat anti-mouse Igs Fc specific capture antibody (anti IgG1, IgG2a, IgG2b, IgG3, IgA, IgM) and goat anti-mouse light chain (kappa and lambda) capture antibody. A mouse monoclonal antibody sample applied to the wells can be isotyped within two hours. Results can be evaluated qualitatively by visual inspection or quantitatively by measuring the absorbance at 450 nm (i.e. if using a goat anti-mouse HRP conjugate as detector).
Features of mouse monoclonal antibody isotyping coated plates:
eight well strip format allows a convenient partial use of the plate; use one strip (column) for each sample (12 samples per plate);
characterize specific antibodies for six different subclasses and two different light-chain types;
accurate specificity characterization with samples containing at least 0.05-0.1 µg/ml of IgG1 – IgG2a – lgG2b – IgG3 antibody and 0.1 – 0.2 µg/ml of IgM – IgA antibody;
high compatibility using samples coming from hybridoma cell culture supernatant, ascites fluid or purified antibodies;
no special equipment is needed to process the microplate; assess results qualitatively evaluated visually or measured quantitatively using an ordinary ELISA plate reader