Coated 96-Well Plates for Mouse Monoclonal Antibodies Isotyping enable easy and accurate identification of mouse immunoglobulin by ELISA Assay
An important step during the production of a monoclonal antibody is the knowledge of its isotype, since it allows to optimize the choice of the method of its purification.Isotyping helps the choice because determines the class (e.g. IgG vs IgA) and subclass (e.g. IgG2a vs IgG2b) of a monoclonal antibody.
Biomat Mouse Monoclonal Antibody Isotyping plate enables easy identification of mouse immunoglobulin class, subclass and light chain. The microplate can be used performing an ELISA strip-well plates with individual wells precoated with goat anti-mouse Igs Fc specific capture antibody (anti IgG1, IgG2a, IgG2b, IgG3, IgA, IgM) and goat anti-mouse light chain (kappa and lambda) capture antibody. A mouse monoclonal antibody sample applied to the wells can be isotyped within two hours. Results can be evaluated qualitatively by visual inspection or quantitatively by measuring the absorbance at 450 nm (i.e. if using a goat anti-mouse HRP conjugate as detector).