---
title: "High Binding 96-Well Plates — 400-500 ng IgG/cm²"
canonical: "https://www.biomat.it/product/microplates/96-well-plates/high-binding-96-well-plates/"
description: "Hydrophilic high binding polystyrene plates, 400-500 ng IgG/cm², CV <5%, for ELISA, CLIA, LIA, FIA. Clear/white/black, 3 formats. ISO 9001, made in Italy."
type: "product"
category: "Microplates / 96-well plates"
manufacturer: "Biomat S.r.l."
updated: "2026-07"
---

# High Binding 96-Well Plates

Optimised for high-sensitivity immunoassays. A hydrophilic surface designed for passive adsorption of proteins, with a binding capacity of **400 to 500 ng IgG/cm²**. Available in clear, white and black polystyrene, in breakable strip, strip and solid formats. Suitable for ELISA, Luminescence (LIA), Fluorescence (FIA) and Chemiluminescent (CLIA) assays.

This is Biomat's flagship surface: the company launched its first proprietary medium and high binding microplates in 1995 and has manufactured them continuously since.

## Specifications

| Parameter | Value |
| --- | --- |
| Material | Polystyrene, pure, low fluorescence |
| Bottom | Flat |
| Surface | High binding capacity, hydrophilic |
| Binding capacity | ~400–500 ng IgG/cm² |
| Selectivity | High affinity even at low protein concentrations (< 50 ng/cm²) |
| Uniformity | CV < 5% |
| Well volume | Breakable strip 350 µl · Strip 360 µl · Solid 400 µl |
| Recommended working volume | 75–300 µl |
| Storage | Room temperature |
| Shelf life | 5 years |
| Dimensions | SBS standard |
| Compatibility | Most common automation systems |
| Unit / minimum order | 50 plates · Packaging 25 per pack |
| Quality | Certificate of Quality released for every lot |

## Key features

- **Hydrophilic high binding surface:** passive adsorption of proteins of varying hydrophilicity; highly selective even at low protein concentrations.
- **Low-fluorescence, high-quality polystyrene:** minimises background noise, improves optical clarity.
- **Colours:** clear, white, black. Customisable upper rim colours on request. White and black reduce well-to-well crosstalk; clear is ideal for spectrophotometric readings.
- **Automation-compatible**, alphanumeric coding for easy well recognition.
- **Ready to use.**

## Formats

- **96-Well Breakable Strip Plates** — 12 breakable strips of 8 wells on a single-well holding frame; strips and single wells can be broken off and repositioned, minimising waste.
- **96-Well Strip Plates** — 12 strips of 8 wells on a rigid 12×8 frame; strips enter in one orientation only, eliminating misorientation.
- **96-Well Solid Plates** — solid format for high-throughput assays; best quality/price ratio.

## Applications

- **ELISA:** competitive and sandwich formats, detecting targets such as steroid hormones and antibodies
- **Luminescence (LIA):** high-sensitivity luminescence detection (white plates)
- **Fluorescence (FIA):** strong signal with minimal interference (black plates)
- **Chemiluminescent (CLIA):** highly sensitive quantitative analyses (white plates)

## High binding or medium binding?

| | High Binding | [Medium Binding](https://www.biomat.it/product/microplates/96-well-plates/medium-binding-96-well-plates/) |
| --- | --- | --- |
| Surface | Mixed hydrophobic/hydrophilic (chemically modified) | Purely hydrophobic polystyrene |
| Binding capacity | 400–500 ng IgG/cm² | 100–200 ng IgG/cm² |
| Interactions | Ionic bonds, van der Waals forces | Hydrophobic interactions |
| Best for | Hydrophilic biomolecules | Hydrophobic biomolecules > 10 kDa |

Biomat has published [experimental adsorption data](https://www.biomat.it/applications-techniques/medium-and-high-binding-well-plates/) comparing the two surfaces on BSA, human IgG and poly-DL-alanine.

## FAQ

**What is the difference between medium binding and high binding microplates?**
Medium binding plates are purely hydrophobic polystyrene, interacting mainly through hydrophobic interactions. High binding plates have a mixed hydrophobic/hydrophilic surface from chemical modification, enabling stronger adsorption through ionic bonds and Van der Waals forces.

**What are the specific advantages of high binding 96-well plates for ELISA?**
The surface is chemically modified to include negatively charged hydrophilic groups within the hydrophobic polystyrene matrix; this blend enhances adsorption of positively charged groups on proteins, giving greater sensitivity and consistency in immobilisation.

**How do high binding plates compare to medium or no binding plates?**
High binding suits hydrophilic biomolecules; medium binding suits hydrophobic ones; no binding plates are treated to prevent any adsorption.

**Can high binding plates be used with non-protein samples?**
Yes, though their enhanced adsorption may increase nonspecific binding, so suitability depends on sample characteristics and assay goals.

**Recommended storage conditions?**
Dry environment, protected from direct sunlight, preferably at room temperature.

## Related

- [Medium Binding 96-Well Plates](https://www.biomat.it/product/microplates/96-well-plates/medium-binding-96-well-plates/)
- [High Binding Capacity surface](https://www.biomat.it/surfaces/high-binding-capacity-surfaces/)
- [Medium vs High Binding: adsorption test data](https://www.biomat.it/applications-techniques/medium-and-high-binding-well-plates/)
- [ELISA Assay technical guide](https://www.biomat.it/applications-techniques/elisa-assay/)
- [Custom Product Development (OEM)](https://www.biomat.it/services/custom-microplates/)

For Research Use Only.

## Manufacturer

Biomat S.r.l., Via Trento 124, 38061 Ala (TN), Italy. ISO 9001:2015 certified. Founded 1992. Contact: info@biomat.it | +39 0464 357951 | [Request a quote](https://www.biomat.it/request-quote/)