Special products

MULTIPLE & PORTABLE ELISA

Patents: EP 1 499 894 B1 – USPTO 7,510,687 – ZL 03 8 10029.0 – PCT/IT03/00218 – WO 03/085401

Limited Use
Unless otherwise expressly authorized in writing, Products are supplied for research use only.
The buyer cannot sell or otherwise transfer products or materials made using Products to a third party or otherwise use products or
materials made using Products for Commercial Purposes, intended as any activity by a party that may include, but is not limited to: use of the Products in manufacturing; use of the Products to provide a service; use of the Products for diagnostic purposes; resale of the Products, whether or not such Products are resold for use in research.
GoDiagnostics srl will not assert a claim against the buyer of infringement of the above patents based upon the manufacture, use or sale of clinical diagnostic, vaccine or prophylactic products developed in research by the buyer in which Products were employed.
For information on purchasing a license for Products for purposes other than research, contact GoDiagnostics srl, Via F. De Sanctis, 86100 Campobasso, Italy – www.god.unimol.it

The innovation and its advantages

MULTIPLE & PORTABLE ELISA is based on patented innovative method and device:

the multi-catcher device with 8 or 12 immunosorbent ogival pins, held in a special rack suitable for moving 8 or 12 multi-catcher devices, easily and simultaneously.

  • Device pins are sensitized with different catchers and different tests are carried out in one shot.
  • No Lab equipments are needed for:
    distributing samples: the multi-catcher device is immerged and incubated in the tube where the sample was collected;
    carrying out washing steps: washing can be made using micro-shower, dispenser, tap water or pre-filled microplates;
    distributing conjugate and chromogenic substrate: pre-filled 96 well plates with the needed reagents are used, sealed and stored until their use;

    • kits for 8 or 12 samples contain 1 microplate pre-filled with conjugate and 1 microplate pre-filled with chromogenic substrate;
    • kits for single sample contain only 1 microplate pre-filled with washing solution, conjugate and chromogenic substrate in different columns.
  • Sample enrichment and sample concentration are not needed: the
    tested sample aliquot increases, easing the highly diluted target detection.

SYSTEM COMPONENTS

System componentsSystem componentsSystem componentsSystem components
8 or 12 pins combrack for combs96 well plate with 12 x 8 pin combs96 well plate with 8 x 12 pin combs

PERFORMING THE TEST

Special product test - 11. dip a multi-catcher device in the collected sample and incubate

extract the device and wash it
Special product test - 22. assemble the devices in the rack, and dip the pins into the conjugate contained in a pre-filled microplate and incubate

extract the rack and wash the pins
Special product test - 33. dip the pins into the chromogenic substrate contained in a pre-filled microplate and incubate

then take out the rack and...
Special product test - 44. ...DONE!!! Your multiple tests are ready for reading contained in a pre-filled microplate and incubate

Reading can be performed visually, for screening purposes, or using the commercially available ELISA-readers (TMB: λ = 655 nm; λ = 450 nm when stop solution is added)

MULTIPLE & PORTABLE ELISA APPLICATIONS

Beyond the laboratory boundaries, cheaply

MULTIPLE & PORTABLE ELISA is specially fitted for optimizing and commercializing:

  • ELISA ready-to-use lab kits
  • ELISA on-site kits

ELISA ready-to-use lab kits and ELISA on-site kits don’t need skilled personnel and lab equipments, so that:

  • cheap labs can be spread widely in:
    – not served areas
    – small industries
    – Med and Vet Offices
  • large population can be screened in situ during:
    – sanitary alerts
    – sport competitions
    – epidemiological surveys in not well established economies
    – duty controls

in the aim of:

  • reaching new end-users
  • extending screening applications

MULTIPLE & PORTABLE ELISA

APPLICATIONS
In a global scenario, the need of increasing controls can be satisfied joining easiness and handling to the ELISA high sensitivity and specificity: ready-to-use lab kits and on-site kits based on MULTIPLE & PORTABLE ELISA are suitable in:

HUMAN HEALTH

  • Breathing Infectious Diseases
  • Sexually Transmitted Infectious Diseases
  • Enteric Infectious Diseases
  • Sanitary Alerts
  • Cancer markers
  • Anti-Doping

FOOD SAFETY

  • Pathogens
  • Allergens
  • Bacterial Toxins
  • Mycotoxins
  • Anabolic agents
  • Antibiotics
  • Pesticides
  • GMO
  • Species identification

ANIMAL HEALTH

  • Anti-Doping (racecourse)
  • Animal Infectious Diseases and Zoonoses (livestock)
  • Animal Infectious Diseases and Zoonoses (pets)

PLANT CULTIVATION

  • Plant Infectious Diseases
  • GMO

WATER QUALITY

  • Pesticides
  • Pathogens
  • Bioterrorism agents