---
title: "Anti-Human IgG, IgM & IgA Coated Surfaces (Fc-Specific)"
canonical: "https://www.biomat.it/surfaces/anti-human-igg-igm-iga/"
description: "Goat anti-human Fc-specific coated surfaces for capture of human IgG, IgM and IgA. Full isotype range from one manufacturer. Binding capacity and sensitivity data."
type: "surface"
manufacturer: "Biomat S.r.l."
updated: "2026-07"
---

# Anti-Human IgG / IgM / IgA Coated Surfaces

Biomat's Goat Anti-Human IgG/IgM/IgA Coated Surfaces are engineered for the specific capture and quantification of human immunoglobulins in biological samples such as serum, plasma, urine, cerebrospinal fluid and saliva.

Each surface is coated with affinity-purified goat anti-human antibodies targeting the Fc region of IgG (Fcγ), IgM (Fcμ) or IgA (Fcα). They are designed as the solid support for sandwich ELISA assays where low background and high sensitivity are required.

Biomat supplies the full range of three isotypes from a single manufacturing source, with lot-to-lot consistency guaranteed by a Certificate of Quality per lot.

## Surface characteristics

- **Fc-specific binding:** IgG via the γ chain, IgM via the μ chain, IgA via the α chain.
- **High specificity:** minimal cross-reactivity with other human immunoglobulin classes and with heterologous serum proteins (mouse, bovine, rabbit).
- **Reliable quantification** of low-abundance human antibodies.
- **Coating volume:** 100 µl/well for all three isotypes, optimised for high signal-to-background ratio.
- **Uniformity:** CV < 10% in ELISA format with HRP-conjugated anti-human antibodies and TMB substrate.

## Specifications by isotype

| Isotype | Target | Coating | Binding capacity | Sensitivity |
| --- | --- | --- | --- | --- |
| Anti-Human IgG | Fcγ | 100 µl/well | 50 ng/well human IgG | 0.1 ng/well |
| Anti-Human IgM | Fcμ | 100 µl/well | ≤ 300 ng/well human IgM | 10 ng/ml |
| Anti-Human IgA | Fcα | 100 µl/well | ≤ 100 ng/well human IgA | 0.700 ng/ml |

### Goat Anti-Human IgG (Fcγ)

An affinity purified goat anti-human IgG that specifically binds the Fc region of human IgG, with minimal cross-reaction to human IgM and IgA and to mouse, bovine and rabbit serum proteins. Used as solid support for sandwich ELISA with a goat anti-human IgG (H+L)-HRP detection antibody.

Binding capacity method: plate saturated with human IgG at 0.500 µg/ml (50 ng/well), ELISA format, goat anti-human IgG (H+L)-HRP detector, TMB substrate. Result: 50 ng/well of human IgG.
Sensitivity: 0.1 ng/well of human IgG, detected significantly above background in the same format.

Technical note NT52: https://www.biomat.it/wp-content/uploads/2025/01/Goat-anti-Human-IgG-coated-surface-TN-52.pdf
Product: [Goat Anti-Human IgG Plates](https://www.biomat.it/product/microplates/96-well-plates/goat-anti-human-igg-plates/)

### Goat Anti-Human IgM (Fcμ)

An affinity purified goat anti-human IgM that specifically binds the Fc region of human IgM, with minimal cross-reaction to human IgG and IgA and to mouse, bovine and rabbit serum proteins. Used as solid support for sandwich ELISA with a goat anti-human IgM (H+L) detection antibody.

Binding capacity method: plate saturated with human IgM at approximately 3.0 µg/ml (300 ng/well), ELISA format, goat anti-human IgM (H+L)-HRP detector, TMB substrate. Result: ≤ 300 ng/well of human IgM.
Sensitivity: 10 ng/ml of human IgM.

Technical note NT54: https://www.biomat.it/wp-content/uploads/2025/01/Goat-anti-Human-IgM-coated-surface-TN-54.pdf
Product: [Goat Anti-Human IgM Plates](https://www.biomat.it/product/microplates/96-well-plates/goat-anti-human-igm-plates/)

### Goat Anti-Human IgA (Fcα)

An affinity purified goat anti-human IgA that specifically binds the Fc region of human IgA, with minimal cross-reaction to human IgG and IgM and to mouse, bovine and rabbit serum proteins. Used as solid support for sandwich ELISA with a goat anti-human IgA (H+L) detection antibody.

Binding capacity method: plate saturated with human IgA at approximately 1.0 µg/ml (100 ng/well), ELISA format, goat anti-human IgA (H+L)-HRP detector, TMB substrate. Result: ≤ 100 ng/well of human IgA.
Sensitivity: 0.700 ng/ml of human IgA.

Technical note NT53: https://www.biomat.it/wp-content/uploads/2025/01/Goat-anti-Human-IgA-coated-surface-TN-53.pdf
Product: [Goat Anti-Human IgA Plates](https://www.biomat.it/product/microplates/96-well-plates/goat-anti-human-iga-plates/)

## Main features

- Ready to use
- Manufactured under ISO 9001 guidelines
- Made in pure polystyrene with low fluorescence
- Post-coated (blocked) for low non-specific binding and long-term stability
- Alphanumeric coding for easy well recognition
- SBS-compliant, suitable for automated processing
- Lot-specific Certificate of Quality supplied
- For Research Use Only

Storage: 2-8°C unopened in original packaging. Once opened, keep sealed with desiccant and use before the expiration date.

## Typical applications

Detection and quantification of human immunoglobulins; replacement of an in-house manual coating protocol with a validated pre-coated solution to reduce experimental variability; isotype-resolved assays where the full IgG/IgM/IgA range is needed from a single supplier with consistent lot performance.

## Related

- [ELISA Assay technical guide](https://www.biomat.it/applications-techniques/elisa-assay/)
- [Choosing the Right Microplate](https://www.biomat.it/applications-techniques/choosing-microplate-for-assay/)
- [Custom Product Development (OEM)](https://www.biomat.it/services/custom-microplates/) — bespoke anti-human coatings and formats

## Manufacturer

Biomat S.r.l., Via Trento 124, 38061 Ala (TN), Italy. ISO 9001:2015 certified. Founded 1992. Contact: info@biomat.it | +39 0464 357951 | [Request a quote](https://www.biomat.it/request-quote/)