High binding capacitsurfaces are used in assays where the adsorbed molecule exceeds (up to 400 ng/cm2) the complementary molecules that have to be detected.
Furthermore, this surface is highly selective and shows high affinity towards the adsorption of molecules also when those are present in very small amounts (<50 ng/cm2) allowing to obtain the maximum sensitivity of the test.
High binding surfaces have different possible fields of application:
- Protein – Antibody Anti-IgM adsorbed onto solid phase used in IgM capture assays
- Lipo-protein – Rubella antigen adsorbed onto solid phase used in IgG assays
- ELISA (competitive) tests for Steroid Hormones and TSH
Microplates show a CV% less than 5 using:
- HIgG as a coating molecule in an ELISA format, anti HIgG-HRP as detector and TMB as substrate
- Biotinylated albumin as a coating molecule in an ELISA format, streptavidin-HRP as detector and TMB as substrate
The microplates are stored at room temperature in sealed PE bags.