A hydrophobic surface suitable for passive adsorption of proteins with different grades of hydrophobicity.
It is used in assays in which the adsorbed molecule present large hydrophobic regions, such as antibodies.
Furthermore this surface is highly selective and shows high affinity towards hydrophobic polypeptides that present a molecular weight > 10kDa.
Here the test carried out for checking the properties of our Medium Binding capacity when IgG were coated on its surface.
Microplates show a CV% less than 5 using:
- HIgG as a coating molecule in an ELISA format, anti HIgG-HRP as detector and TMB as substrate
- Biotinylated albumin as a coating molecule in an ELISA format, streptavidin-HRP as detector and TMB as substrate
The microplates are stored at room temperature in sealed PE bags.