Poly-L-Lysine Coated Surface can be used to exploit their high density of cationic groups to react through electrostatic and stereospecific bonds.
Poly-L-Lysine Coated Surface is a surface obtained using a synthetic molecule rich in α amino, α carboxyl, ε amino groups without that polystyrene optical features are modified. These groups are able to react through electrostatic and stereospecific bonds and allow the modified surface to be used as a valid tool to carry out biological tests.
Examples of applications for Poly-L-Lysine Coated surface
- interactions with plasminogen and plasminogen activator
- interactions with ribosomial RNA
- interactions with double stranded DNA
Poly-L-lysine Coated Surfaces are also ideally suited for the adhesion of cells, since the cell surface is known to be negatively charged.
Biomat Poly-L-Lysine Coated Products
Poly-L-Lysine Coated Surfaces main features
Biomat Poly-L-Lysine Coated products have the following characteristics:
- Ready to Use
- Manufactured under ISO:9001 guidelines
- All lots are tested for uniformity and reproducibility
- Certificate of Quality is released for every lot
- For Research Use Only
Also, Poly-L-Lysine Coated Products ensures:
The Poly-L-Lysine Coated Products show a CV% less than 5 when used as a catcher of NHS-biotin in an ELISA format, using Streptavidin-HRP as detector and TMB as substrate.
Storage and Stability
The Poly-L-Lysine Coated products, if unopened, are stable at 2 – 8 °C until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.
Poly-L-Lysine Coated Microplates Specifications
Poly-L-Lysine (M.W. 70 – 150 kDa) is coated using 200 μl/well.
Biomat shows a test suitable for measuring the available ε- amino groups on Lysine. Click on the following link to see a general procedure for binding NHS-biotin to Poly-L-Lysine Coated 96 Well Plate.
Poly-L-Lysine coated wells were incubated with dsDNA molecule solution in an ELISA format using human IgG anti-DNA as a catcher, anti HIgG-HRP as detector and TMB as substrate.
Click on the following link to see the results.