Poly-L-Lysine Coated Surface can be used to exploit their high density of cationic groups to react through electrostatic and stereospecific bonds.

Poly-L-Lysine Coated Surface is a surface obtained  using a synthetic molecule rich in α amino, α carboxyl, ε amino groups without that polystyrene optical features are modified. These groups are able to react through electrostatic and stereospecific bonds and allow the modified surface to be used as a valid tool to carry out biological tests.

Examples of applications for Poly-L-Lysine Coated surface

  • interactions with plasminogen and plasminogen activator
  • interactions with ribosomial RNA
  • interactions with double stranded DNA

Poly-L-lysine Coated Surfaces are also ideally suited for the adhesion of cells, since the cell surface is known to be negatively charged.

Biomat Poly-L-Lysine Coated Products

Poly-L-Lysine Coated Surfaces main features

Biomat Poly-L-Lysine Coated products have the following characteristics:

  • Ready to Use
  • Manufactured under ISO:9001 guidelines
  • All lots are tested for uniformity and reproducibility
  • Certificate of Quality is released for every lot
  • For Research Use Only

Also, Poly-L-Lysine Coated Products ensures:

Uniformity

The Poly-L-Lysine Coated Products show a CV% less than 5 when used as a catcher of NHS-biotin in an ELISA format, using Streptavidin-HRP as detector and TMB as substrate.

Storage and Stability

The Poly-L-Lysine Coated products, if unopened,  are stable at 2 – 8 °C until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.

Poly-L-Lysine Coated Microplates Specifications

Coating

Poly-L-Lysine (M.W. 70 – 150 kDa)  is coated using 200 μl/well.

Binding activity

Biomat shows a test suitable for measuring the available ε- amino groups on Lysine. Click on the following link to see a general procedure for binding NHS-biotin to Poly-L-Lysine Coated 96 Well Plate.

Stereospecific Binding Activity technical note

Functional activity

Poly-L-Lysine coated wells were incubated with dsDNA molecule solution in an ELISA format using human IgG anti-DNA as a catcher, anti HIgG-HRP as detector and TMB as substrate.

Click on the following link to see the results.

Electrostatic Functional Activity technical note