The monomeric L-Lysine chain shows a high density of groups:

  • α-amino
  • α-carboxyl
  • ε-amino

These groups are able to react through electrostatic and stereospecific bonds. The polystyrene optical features don’t change, allowing the modified surface to be used as a valid tool to carry out biological tests.
This surface shows its usefulness for these applications:

  • interactions with plasminogen and plasminogen activator
  • interactions with ribosomial RNA
  • interactions with double stranded DNA
Product specifications

Available configurations

96-well microplates, solid or with 12 removable 8-well strips.

Coating

Poly-L-Lysine is coated using 200 µl/well. The strips are post-coated (blocked) for low non specific binding and long-term stability.

Binding activity

Poly-L-Lysine coated wells were incubated with NHS-biotin solution in an ELISA format using Streptavidin-HRP as detector and TMB as substrate.

see Stereospecific Binding Activity technical note

Functional activity

Poly-L-Lysine coated wells were incubated with dsDNA molecule solution in an ELISA format using human IgG anti-DNA as a catcher, anti HIgG-HRP as detector and TMB as substrate.

see Electrostatic Functional Activity technical note

Uniformity

Microplates show a CV% less than 5 when are used as a catcher of NHS-biotin in an ELISA format, using  Streptavidin-HRP as detector and TMB as substrate.

 

Storage and Stability

The microplates, if unopened, are stable refrigerated until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.