---
title: "Streptavidin Coated Surface for Biotin Binding"
canonical: "https://www.biomat.it/surfaces/streptavidin-coated-surfaces/"
description: "How Biomat's streptavidin coating captures biotinylated molecules: tetrameric binding, 12 pmol biotin/well, low background. Technical data and applications."
type: "surface"
manufacturer: "Biomat S.r.l."
updated: "2026-07"
---

# Streptavidin Coated Surface

Streptavidin Coated Surface is a universal capture platform for any biotinylated molecule: antibodies, antigens, proteins, peptides, polysaccharides, oligonucleotides and DNA fragments.

Streptavidin is a tetrameric protein (MW 60 kDa) with very high affinity for biotin (affinity constant on the order of 10^15 M^-1; Kd approximately 10^-15 M). The streptavidin-biotin bond is the strongest known non-covalent biological interaction. Each of the four streptavidin subunits binds one biotin molecule.

## When to use this surface

Streptavidin coating is the solution for molecules that do not bind reliably by passive adsorption, or that adsorb in an unfavourable orientation masking their active sites. Biotin is small enough to be conjugated to most proteins without altering their activity, and each protein can carry several biotin molecules. The result is oriented, stable capture with high specificity and low background, which raises sensitivity in ELISA, nucleic acid capture and other biochemical assays.

## Microplate specifications

| Parameter | Value |
| --- | --- |
| Coating volume | 200 µl/well |
| Binding capacity, biotin | 12 pmol/well (200 µl volume) |
| Binding capacity, biotinylated human IgG | 100 ng/well (100 µl volume) |
| Uniformity | CV < 5% (biotin-HRP detector, TMB substrate) |
| Storage | 2-8°C unopened, until expiration date |

Binding capacity method (biotin): streptavidin coated wells and BSA-saturated control wells were incubated with a calibrated biotin solution; aliquots were mixed with biotinylated peroxidase and transferred to new streptavidin coated wells; well capacity was derived from the enzyme bound to the solid phase, corrected for non-specific binding to control wells.

Binding capacity method (biotinylated HIgG): ELISA format, HIgG-HRP detector, TMB substrate.

## PCR 8-strip tubes and PCR plates specifications

| Parameter | Value |
| --- | --- |
| Coating volume | 100 µl/tube |
| Binding capacity, biotin | approximately 5-6 pmol d-biotin/tube |

Method: incubation with biotin solutions containing biotinylated peroxidase, 30 min RT; after washing, TMB incubation, blocked with 1N sulphuric acid, OD read at 450 nm.

## Stability data

- **Long storage:** coated wells held for 30 months in a warehouse without air conditioning (10°C to 40°C), compared against samples stored at 4°C. The coating remained stable.
- **Shelf life at 37°C:** coated wells held 15 days at 37°C compared against a 4°C standard, tested with biotinylated IgG at 100 ng/ml.
- **Temperature stress (transport simulation):** 4°C for 10 days; 22-23°C for 10 days; and a cycle of 37°C 3 days / 22-23°C 12 hours / -20°C 10 hours / 37°C 3 days / 22-23°C 3 days.
- **Endurance under strong chemical contacts:** tested by substituting the first washing step with incubations in SDS 0.1% in 0.6M NaCl (56°C, 2h); 30% formamide in 0.6M NaCl (37°C, 2h); 0.1% Tween 20 in 0.1M PBS (37°C, 2h); 0.05M NaOH (RT, 20 min); 0.2M NaOH (RT, 20 min); urea 8M (5 to 60 min).

## Main features

- Ready to use
- Manufactured under ISO 9001 guidelines
- Post-coated (blocked) for low non-specific binding and long-term stability
- All lots tested for uniformity and reproducibility
- Certificate of Quality released for every lot
- For Research Use Only

## Products with this surface

- [Streptavidin Plates, 96-well](https://www.biomat.it/product/microplates/96-well-plates/streptavidin-plates/)
- [Streptavidin PCR 8 Strip Tubes](https://www.biomat.it/product/pcr-products/coated-pcr-strip-tubes/streptavidin-coated-pcr-tubes/)
- [Streptavidin PCR Plates](https://www.biomat.it/product/pcr-products/coated-pcr-plates/streptavidin-pcr-plates/)

## Related

- [Streptavidin High Binding Coated Surface](https://www.biomat.it/surfaces/streptavidin-high-binding-coated-surfaces/) — for biotinylated low molecular weight molecules
- [ELISA Assay technical guide](https://www.biomat.it/applications-techniques/elisa-assay/)
- Technical note, binding capacity and specificity: https://www.biomat.it/wp-content/uploads/2018/02/STREPTAVIDIN-COATED-SURFACE-TN-7-1_3.pdf
- Technical note, stability tests: https://www.biomat.it/wp-content/uploads/2018/02/STREPTAVIDIN-COATED-SURFACE-TN-7-5.pdf

## Manufacturer

Biomat S.r.l., Via Trento 124, 38061 Ala (TN), Italy. ISO 9001:2015 certified. Founded 1992. Contact: info@biomat.it | +39 0464 357951 | [Request a quote](https://www.biomat.it/request-quote/)