---
title: "Streptavidin High Binding Coated Surface"
canonical: "https://www.biomat.it/surfaces/streptavidin-high-binding-coated-surfaces/"
description: "Polymeric streptavidin surface with >35 pmol d-biotin/well, designed for measuring biotinylated low molecular weight molecules. Technical data and applications."
type: "surface"
manufacturer: "Biomat S.r.l."
updated: "2026-07"
---

# Streptavidin High Binding Coated Surface

Streptavidin High Binding Coated Surface captures any biotinylated molecule (proteins, peptides, polysaccharides, oligonucleotides, DNA fragments) and is specifically designed for measuring biotinylated low molecular weight molecules.

High binding streptavidin is a polymer of 6-8 streptavidin molecules, which offers more biotin-binding sites per well than standard streptavidin. Since each streptavidin subunit binds one biotin molecule, the higher site density translates directly into greater assay sensitivity.

## Difference from the standard streptavidin surface

Unlike the standard Streptavidin Coated Surface, the high binding variant is particularly useful in competitive tests and indirect assays measuring biotinylated low molecular weight molecules, where the higher density of binding sites delivers greater sensitivity and stability. For general capture of biotinylated proteins and antibodies, the standard streptavidin surface is normally sufficient.

| | Streptavidin | Streptavidin High Binding |
| --- | --- | --- |
| Composition | Tetrameric protein, 60 kDa | Polymer of 6-8 streptavidin molecules |
| Coating volume | 200 µl/well | 100 µl/well |
| Binding capacity, d-biotin | 12 pmol/well | > 35 pmol/well |
| Binding capacity, HIgG | 100 ng/well | ~100 ng/well |
| Best for | General biotinylated capture | Biotinylated low MW molecules, competitive assays |

## Microplate specifications

| Parameter | Value |
| --- | --- |
| Coating volume | 100 µl/well |
| Binding capacity, small molecules (d-biotin) | > 35 pmol d-biotin/well |
| Binding capacity, large molecules (human IgG) | approximately 100 ng/well |
| Uniformity | CV < 5% (biotin-HRP detector, TMB substrate) |
| Storage | 2-8°C unopened, until expiration date |

Methods: small molecules, ELISA format with biotinylated peroxidase detector and TMB substrate. Large molecules, ELISA format with biotinylated HIgG, HIgG-HRP detector, TMB substrate.

## PCR 8-strip tubes and PCR plates specifications

| Parameter | Value |
| --- | --- |
| Coating volume | 100 µl/tube |
| Binding capacity, small molecules (d-biotin) | > 20 pmol d-biotin/tube |

Method: incubation with biotin solutions containing biotinylated peroxidase, 30 min RT; after washing, TMB incubation, blocked with 1N sulphuric acid, OD read at 450 nm.

## Main features

- Ready to use
- Manufactured under ISO 9001 guidelines
- All lots tested for uniformity and reproducibility
- Certificate of Quality released for every lot
- For Research Use Only

Storage: unopened, stable at 2-8°C until the expiration date on the label. Once opened, store in a closed pouch with desiccant and use within the expiration date.

## Products with this surface

- [High Binding Streptavidin Plates, 96-well](https://www.biomat.it/product/microplates/96-well-plates/high-binding-streptavidin-plates/)
- [Streptavidin High Binding PCR 8 Strip Tubes](https://www.biomat.it/product/pcr-products/coated-pcr-strip-tubes/high-binding-streptavidin-high-pcr-8-strip-tubes/)
- [Streptavidin High Binding PCR Plates](https://www.biomat.it/product/pcr-products/coated-pcr-plates/streptavidin-high-binding-pcr-plates/)

## Related

- [Streptavidin Coated Surface](https://www.biomat.it/surfaces/streptavidin-coated-surfaces/) — the standard variant
- [ELISA Assay technical guide](https://www.biomat.it/applications-techniques/elisa-assay/)
- Technical note, binding capacity small molecules: https://www.biomat.it/wp-content/uploads/2018/02/STREPTAVIDIN-HB-COATED-SURFACE-TN-31a.pdf
- Technical note, binding capacity large molecules: https://www.biomat.it/wp-content/uploads/2018/02/STREPTAVIDIN-HB-COATED-SURFACE-TN-31b.pdf
- Technical note, PCR binding capacity: https://www.biomat.it/wp-content/uploads/2018/02/STREPTAVIDIN-HB-COATED-PCR-TN-33.pdf

## Manufacturer

Biomat S.r.l., Via Trento 124, 38061 Ala (TN), Italy. ISO 9001:2015 certified. Founded 1992. Contact: info@biomat.it | +39 0464 357951 | [Request a quote](https://www.biomat.it/request-quote/)