Streptavidin High Binding

Streptavidin High Binding Coated Surface offers a powerful and universal instrument for binding any biotinylated molecule, mostly for measuring biotinylated low molecular weight molecules in competitive tests.

HB streptavidin coated surfaces are a powerful and universal instrument for binding any biotinylated molecule (Proteins-Peptides-Polysaccharides-Oligonucleotides-DNA fragments-etc.).
Biotin is a small molecule which can be conjugated to many proteins without loosing or altering their activity, each protein can bind many biotin molecules.
Since each subunit of streptavidin binds one molecule of biotin, the resulting effect is a great increase of the sensitivity of the assay.
Unlike the normal Streptavidin coated, these surfaces are particularly useful in competitive tests to measure biotinylated low molecular weight molecules.

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96 Well Plates:

• Streptavidin High Binding 96 Well Breakable Strip Plates
• Streptavidin High Binding 96 Well Strip Plates
• Streptavidin High Binding 96 Well Solid Plates

PCR Products

• Streptavidin High Binding PCR 8 strip tubes
• Streptavidin High Binding PCR plates

Special Products

• Streptavidin High Binding 12×75 mm Tubes

Uniformity

The coated products show a CV% less than 5 using biotin-HRP as detector and TMB as substrate.

Storage and Stability

The coated products, if unopened, are stable at 2-8°C until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.

Coating

HB Streptavidin is coated using 100 µl/well. The strips are post-coated (blocked) for low non specific binding and long-term stability.

Binding capacity for small molecules (e.g. d-biotin)

The HB Streptavidin coated wells were incubated with biotin solutions, in an ELISA format, using biotinylated peroxidase as detector and TMB as substrate.

see Binding Capacity technical note

Binding capacity for big molecules (e.g. HIgG)

The HB Streptavidin coated wells were incubated with biotinylated HIgG in an ELISA format using HIgG-HRP as a detector and TMB as substrate.

see Binding Capacity technical note

Streptavidin High Binding 96 Well Solid Plates

Streptavidin High Binding 96 Well Strip Plates

Streptavidin High Binding 96 Wells Breakable Strip Plates

Coating

HB Streptavidin is coated using 100 µl/tube. The PCR 8 strip tubes and plates are post-coated (blocked) for low non specific binding and long-term stability.

Binding capacity for small molecules (e.g. d-biotin)

HB Streptavidin coated PCR tubes strips were incubated with biotin solutions containing biotinylated peroxidase for 30’ R.T. After the washing step, the PCR tubes strips were incubated with TMB and blocked with sulphuric acid 1N. The O.D. values were read at 450 nm

see Binding Capacity technical note

Streptavidin High Binding PCR 8 strip tubes

Streptavidin High Binding PCR plates

Coating

HB Streptavidin is coated using 500 µl/tube. The tubes 12×75 are post-coated (blocked) for low non specific binding and long-term stability.

Binding capacity towards biotin

HB Streptavidin coated tubes 12×75 were incubated with biotin solutions (from 0 to 195.2 ng/ml) containing 4 ng/ml of biotinylated peroxidase for 30’ R.T. After the washing step, the streptavidin tubes 12×75 were incubated with TMB and blocked with sulphuric acid 1N. The O.D. values were read at 450 nm.

see Binding Capacity technical note

Streptavidin High Binding 12×75 mm Tubes