Streptavidin High Binding

Streptavidin High Binding Coated Surface offers a powerful and universal instrument for binding any biotinylated molecule, mostly for measuring biotinylated low molecular weight molecules in competitive tests.

Streptavidin High Binding Coated Surface is suitable for binding antibodies or probes for ELISA assays and other target-specific assays. This surface is a powerful and universal instrument for binding any biotinylated molecule (Proteins-Peptides-Polysaccharides-Oligonucleotides-DNA fragments-etc.).

Biotin is a small molecule which can be conjugated to many proteins without loosing or altering their activity, each protein can bind many biotin molecules.

Since each subunit of streptavidin binds one molecule of biotin, the resulting effect is a great increase of the sensitivity of the assay.

Unlike the normal Streptavidin coated, these surfaces are particularly useful in competitive tests to measure biotinylated low molecular weight molecules.

96-Well Plates

• Streptavidin High Binding Coated 96-Well Plates
• Streptavidin High Binding Coated 96-Well Breakable Strip Plates
• Streptavidin High Binding Coated 96-Well Strip Plates
• Streptavidin High Binding Coated 96-Well Solid Plates

Other 96-Well Plates

PCR Products

• Streptavidin High Binding Coated PCR 8 Strip Tubes
• Streptavidin High Binding Coated PCR Plates

Other PCR Products

Special Products

• Streptavidin High Binding Coated 12×75 mm Tubes

Other Special Products

Biomat Streptavidin High Binding Coated products have the following characteristics:

• Ready to Use
• Manufactured under ISO:9001 guidelines
• All lots are tested for uniformity and reproducibility
• Certificate of Quality is released for every lot
• For Research Use Only

Also, Streptavidin High Binding Coated Products ensures:

Uniformity

The Streptavidin High Binding Coated Products  show a CV% less than 5 using biotin-HRP as detector and TMB as substrate.

Storage and Stability

The Streptavidin High Binding Coated products, if unopened,  are stable at 2 – 8 °C until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.

Coating

Streptavidin High Binding is coated using 100 µl/well.

Binding capacity for small molecules (e.g. d-biotin)

The Streptavidin High Binding coated wells were incubated with biotin solutions, in an ELISA format, using biotinylated peroxidase as detector and TMB as substrate.

see Binding Capacity technical note

Binding capacity for big molecules (e.g. HIgG)

The Streptavidin High Binding coated wells were incubated with biotinylated HIgG in an ELISA format using HIgG-HRP as a detector and TMB as substrate.

see Binding Capacity technical note

Coating

Streptavidin High Binding is coated using 100 µl/tube.

Binding capacity for small molecules (e.g. d-biotin)

Streptavidin High Binding coated PCR tubes strips were incubated with biotin solutions containing biotinylated peroxidase for 30’ R.T. After the washing step, the PCR tubes strips were incubated with TMB and blocked with sulphuric acid 1N. The O.D. values were read at 450 nm

see Binding Capacity technical note

Coating

Streptavidin High Binding is coated using 500 µl/tube.

Binding capacity towards biotin

Streptavidin High Binding coated tubes 12×75 were incubated with biotin solutions (from 0 to 195.2 ng/ml) containing 4 ng/ml of biotinylated peroxidase for 30’ R.T. After the washing step, the streptavidin tubes 12×75 were incubated with TMB and blocked with sulphuric acid 1N. The O.D. values were read at 450 nm.

see Binding Capacity technical note