The area which is covered by the liquid is that which is involved in the reactions.
At each level the total area is made of the bottom surface, which is the same for every level, and the wall surface, which is variable.
The optical path is another important feature, since it can affect the reading, sometimes giving a false impression of higher or lower sensitivity.
A smaller diameter of the well means that the level of the same quantity of liquid will be higher so that the light beam will pass through a wider extension of coloured liquid. Obviously some other factors as:
– the shape of the meniscus of the liquid
– the exact alignment of the light beam so that it passed at the very center of the well which is the low or higher, depending upon the wettability of the well
– the wettability itself affects this value, so these figures are intended only to be indicative
|Level ml||Optical path mm||Bottom surface mm2||Wall surface mm2||Total surface mm2|