Protein A Coated Plates

Protein A Coated Plates are designed for the capture of IgG applied directly or as antibody/antigen complex.

The Protein A surface of Protein A Coated 96-Well Plates provides alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plates-based assay techniques...
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Protein A specifically binds the Fc region of immunoglobulins of many mammalian species with an orientation that allows the F(ab)2 binding sites to be freely available for efficient binding to epitope.

Examples of applications for Protein A Coated Plates are:

  • specific and sterically oriented bond of IgG;
  • separation of IgG from other immunoglobulins;
  • separation of antigen-antibodies complexes;
  • separation of IgG from contaminants;
  • isolation and analysis of fusion proteins;
  • finding and identifying red cell antibodies (only on U-bottom plates).

Biomat Protein A Coated 96-Well Plates are made in Transparent, White and Black Polystyrene and can be used for quantitative and qualitative immunoassays like ELISA, Chemiluminescent (CLIA) , Luminescence (LIA) and Fluorescence (FIA) assays.

Protein A Coated Plates are pre-blocked in order to minimize any non-specific binding and to ensure long-term stability.

Protein A Coated 96-Well Plates formats

Protein A Coated 96-Well Plates are offered in 3 different formats for maximum flexibility:

The well can have a volume capacity of: 

  • 350 µl,
  • 360 µl,
  • 400 µl.

Biomat Protein A Coated Plates main features

Biomat Protein A Coated 96-Well Plates are suitable with ELISA Assay Technology and others immunoassays due to these features:

  • they are ready to use
  • they are manufactured under ISO:9001 guidelines
  • they are made in pure polystyrene with low fluorescence
  • the mould design provides optical quality, important to reduce the background signal
  • the wells inner bottom radius edge improves the efficiency of washings
  • the external lid warrants vertical alignment when using single microplate wells
  • they are equipped with alphanumeric coding for easy well recognition
  • the rim protects the external face of the bottom from scratches
  • the microplates comply with SBS standards and the design assures a good performance in automatic processing plant
  • all lots are tested for uniformity and reproducibility
  • the Certificate of Quality is released for every lot

Protein A Surface