Goat Anti Human IgG/IgM/IgA coated surface
Biomat’s Goat Anti-Human IgG/IgM/IgA Coated Surfaces are engineered for the specific capture and quantification of human immunoglobulins in biological samples such as serum, plasma, urine, cerebrospinal fluid, saliva, and others.
Each surface is coated with affinity-purified goat anti-human antibodies, specifically targeting the Fc region of IgG (Fcγ), IgM (Fcμ), or IgA (Fcα). These surfaces are ideal for sandwich ELISA assays, where reliability, low background, and high sensitivity are essential.
Surface Characteristics
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Fc-Specific Binding
Each coating binds selectively to the Fc fragment of the target immunoglobulin:-
IgG via γ chain
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IgM via µ chain
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IgA via α chain
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High Specificity
Minimal cross-reactivity with other immunoglobulins and heterologous serum proteins (e.g. mouse, bovine, rabbit). -
Reliable Quantification
Enables accurate detection of low-abundance human antibodies. -
High Binding Affinity
Surfaces show strong and specific adsorption of target immunoglobulins, enhancing assay performance.
Biomat’s Anti Human IgG/IgM/IgA Coated 96-Well Plates products
These are the available configurations of Anti human IgG/IgM/IgA 96-Well Coated Plates products by Biomat.
Main Features of Biomat’s Anti-Human IgG/IgM/IgA Coated Surface
Biomat Coated Plates with Anti human IgG/IgM/IgA  are suitable with ELISA Assay Technology due to these features:
- Ready to use
- Manufactured under ISO:9001 guidelines
- Made in pure polystyrene with low fluorescence
- Post-coated (blocked) for low non specific binding and long-term stability
- Alphanumeric coding for easy well recognition
- Microplates comply with SBS standards and the design assures a good performance in automatic processing plant
- Supplied with lot-specific Certificate of Quality
- For Research Use Only
Also, Anti human IgG/IgM/IgA Coated 96-Well Plates ensure:
Performance and Uniformity
When used as a capture surface in ELISA format (with HRP-conjugated anti-human antibodies and TMB substrate), Biomat’s Anti-Human IgG/IgM/IgA surfaces exhibit:
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Uniform coating across all wells
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Coefficient of Variation (CV%) < 10%, ensuring assay reproducibility
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High sensitivity, with detection limits well below 1 ng/well (varies by isotype)
Storage and Stability
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Store at 2–8°C unopened, in original packaging
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Once opened, keep sealed with desiccant and use before the indicated expiration date
Available Fc-Specific Surface Variants
Biomat offers Anti-Human Coated Surfaces for the following immunoglobulin classes:
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Anti-Human IgG (Fcγ)
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High specificity for human IgG Fc region
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Minimal cross-reactivity with IgA, IgM, and heterologous sera
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Anti-Human IgM (Fcμ)
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Binds human IgM Fc region with strong affinity
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Low cross-reaction with IgG, IgA
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Anti-Human IgA (Fcα)
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Targets human IgA Fc region
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Minimal interference from other Ig classes
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Each coating is applied at 100 μL/well and optimized for high signal-to-background ratios in ELISA assays.
Anti human IgG/IgM/IgA Coated Surfaces Product specifications
Goat Anti Human IgG Fcγ
An affinity purified goat anti human IgG specifically binds the Fc region of human IgG immunoglobulins, with minimal cross-reaction to human IgM and IgA, mouse, bovine and rabbit serum proteins.
These plates may be used as solid support for sandwich ELISA assay utilizing a goat anti human IgG ( H+L )-HRP detection antibody.
Goat Anti Human IgG Fcγ Coated 96-Well Plates Product Specifications
Coating
Affinity purified goat anti human IgG Fcγ is coated using 100 μl/well.
Binding capacity
Microplate was saturated with human IgG at a concentration of 0.500 μg/ml ( 50 ng/well) in an ELISA format using goat anti human IgG (H+L)-HRP as detector and TMB as substrate.
Sensitivity
Human IgG was detected at a concentration significantly above background in an ELISA format using goat anti human IgG (H+L)-HRP as detector and TMB as substrate.
see Binding capacity and Sensitivity technical note Anti Human IgG (NT52)
Goat Anti Human IgM Fcµ
An affinity purified goat anti human IgM specifically binds the Fc region of human IgM immunoglobulins , with minimal cross reaction to human IgG and IgA, mouse, bovine and rabbit serum proteins.
These plates may be used as solid support for sandwich ELISA assay utilizing a goat anti human IgM ( H+L ) detection antibody.
Goat Anti Human IgM Fcµ Coated 96-Well Plates Product Specifications
Coating
Affinity purified Goat anti human IgM Fcµ is coated using 100 μl/well.
Binding capacity and Sensitivity
Microplate was saturated with human IgM at a concentration of  about 3.0 μg/ml (300 ng/well) in an ELISA format using goat anti human IgM (H+L)-HRP as detector and TMB as substrate.
Sensitivity
Human IgM was detected at a concentration significantly above background in an ELISA format using goat anti human IgM ( H+L )-HRP as detector and TMB as substrate.
see Binding capacity and Sensitivity technical note Anti Human IgM (NT54)
Goat Anti Human IgA Fcα
An affinity purified goat anti human IgA specifically binds the Fc region of human IgA immunoglobulins , with minimal cross reaction to human IgG and IgM, mouse, bovine and rabbit serum proteins.
These plates may be used as solid support for sandwich ELISA assay utilizing a goat anti human IgA ( H+L ) detection antibody.
Goat Anti Human IgA Fcα Coated 96-Well Plates Product Specifications
Coating
Affinity purified Goat anti human IgA Fcα is coated using 100 μl/well.
Binding capacity
Microplate was saturated with human IgA at a concentration of about 1.0 μg/ml (100 ng/well) in an ELISA format using goat anti human IgA (H+L)-HRP as detector and TMB as substrate.
Sensitivity
Human IgA was detected at a concentration significantly above background in an ELISA format using goat anti human IgA ( H+L )-HRP as detector and TMB as substrate.
see Binding capacity and Sensitivity technical note Anti Human IgA (NT53)
