ELISA Coating Buffer

ELISA Coating Buffers allows immobilizing proteins or antibodies on microplates.

Biomat’s ELISA Coating Buffers allow the direct or indirect immobilization of antigens to the surface of polystyrene microplate wells. 

A key factor in immobilization of molecules coating step, can be the pH of the coating buffer in which the biomolecules are dissolved in. Selecting an ELISA coating buffer between pH 6.0 and pH 9.6 can have an effect on the total charge and the steric structure of the biomolecules’ binding and this affects their biomolecules immobilization.

Biomat reagents for ELISA coating include three different ELISA coating buffers to meet everyone’s needs. 

We offer:

  • Phosphate buffer pH 6.0: balanced salt solution. Is one of the most commonly used buffers for adsorptive immobilization of proteins and antibodies on plastic surfaces for ELISA, EIA, RIA, proteins array and immuno-PCR applications. This ELISA coating buffer, at pH 6.0, is specific for proteins or antibodies that prefer a slightly acidic environment; it’s also suitable for cell culture and molecular biology applications. It helps to maintain pH and provides phosphate ions.
  • Phosphate buffer pH 7.2: buffer isotonic and non-toxic to cells and has the ability to maintain osmolarity and for this is used for cell culture applications (for example washing cells, transporting cells or tissue samples, diluting cells for counting and preparing reagents). ELISA coating buffer pH 7.2 is also often used for sample dilution in molecular biology and as protein diluent in Western blotting.
  • Carbonate/Bicarbonate buffer pH 9.6: ELISA carbonate coating buffer is used for proteins and antibodies coating procedures on plastic surfaces. Carbonate/Bicarbonate Coating Buffer is also used for adsorptive immobilization of other protein binding surfaces for ELISA, EIA, RIA techniques, immuno-PCR and protein arrays. Used extensively in molecular and cell biology.

All three types of ELISA Coating Buffer Reagents can be provided diluted (1X) or concentrated (10X); they can be requested either in liquid or powder form, to be diluted when necessary.

Use our ELISA Coating Buffers  with our 96-Well Plates to develop your ELISA assay by choosing the one best suited to your molecules to attach to the polystyrene wells. 

Discover other reagents typically used in ELISA assays.