Jacalin Coated Surface can be used in assays for binding carbohydrate-binding proteins and glycoproteins.

Jacalin, belonging to the lectins family, is a tetrameric (M.W. 40 kDa) carbohydrate-binding protein isolated from the seeds of jack fruit Artocarpus integrifolia agglutinin. It is well known that lectins have been used extensively for the isolation of glycol-conjugates and glycoproteins with specific carbohydrate structures.

Jacalin shows specific affinity for molecules containing non reducing α-D-galactosyl groups, usually present in the biochemical structure of IgA1 and cell membranes.

Examples of applications for Jacalin Coated surface

  • Human IgA1 specific binding, sterically oriented
  • Purification of human immunoglobulins (especially IgA1)
  • Separation of immunocomplexes antigen-antibody
  • Separation of IgA1 from contaminants
  • Stimulation of T-cells

Biomat Jacalin Coated Products

96-Well Plates

Other 96-Well Plates

Jacalin Coated Surfaces main features

Biomat Jacalin Coated products have the following characteristics:

  • Ready to Use
  • Manufactured under ISO:9001 guidelines
  • All lots are tested for uniformity and reproducibility
  • Certificate of Quality is released for every lot
  • For Research Use Only

Also, Jacalin Coated Products ensures:

Uniformity

The Jacalin Coated Products  show a CV% less than 5 used as a catcher of biotinylated human IgA in an ELISA format using streptavidin-HRP as detector and TMB as substrate.

Storage and Stability

The Jacalin Coated products, if unopened,  are stable at 2 – 8 °C until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.

Jacalin Coated Microplates Specifications

Coating

Jacalin (M.W. 40 kDa) is coated using 100 μl/well.

Binding capacity

Microplate was saturated with biotinylated human IgA at a concentration of 6 μg/ml (600 ng/well) in an ELISA format using Streptavidin-HRP as detector and TMB as substrate.

see Binding Capacity technical note

Result:

~ 4 pmol IgA/well

Sensitivity

Biotinylated human IgA was detected at a concentration significantly above background in an ELISA format using streptavidin-HRP as detector and TMB as substrate.

see Sensitivity technical note

Result:

0.0161 μg/ml (1.61 ng/well) of IgA