Jacalin, belonging to the lectins family, is a tetrameric (M.W. 40 kDa) carbohydrate-binding protein isolated from the seeds of jack fruit Artocarpus integrifolia agglutinin. It is well known that lectins have been used extensively for the isolation of glycol-conjugates and glycoproteins with specific carbohydrate structures.
Jacalin shows specific affinity for molecules containing non reducing α-D-galactosyl groups, usually present in the biochemical structure of IgA1 and cell membranes.
Example of applications:
- Human IgA1 specific binding, sterically oriented
- Purification of human immunoglobulins (especially IgA1)
- Separation of immunocomplexes antigen-antibody
- Separation of IgA1 from contaminants
- Stimulation of T-cells
Jacalin ( M.W. 40 kDa ) , from the seeds of jack fruit Artrocarpus integrifolia, is coated using 100 μl/well. The strips are post-coated ( blocked ) for low non specific binding and long-term stability.
Microplate was saturated with biotinylated human IgA at a concentration of 6 μg/ml (600 ng/well) in an ELISA format using Streptavidin-HRP as detector and TMB as substrate.
~ 4 pmol IgA/well
Biotinylated human IgA was detected at a concentration significantly above background in an ELISA format using streptavidin-HRP as detector and TMB as substrate.
0.0161 μg/ml (1.61 ng/well) of IgA
Microplates show a CV% less than 5 when used as a catcher of biotinylated human IgA in an ELISA format using streptavidin-HRP as detector and TMB as substrate.
Storage and Stability
The microplates, if unopened, are stable refrigerated until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.