Coated Surface for Immunotoxicity ELISA Test is ideal for setting up ELISA IgM and IgG assays to be used as biomarkers of immunotoxicity.

Immunotoxicity is damage to the immune system caused by exposure to substances (chemicals, environmental, drugs, etc.) that are capable of altering either or more immune functions, causing an undesirable effect.

Immunotoxicity analysis play a fundamental role in studying the development of new drugs because hypersensitivity reactions are the most frequent immunotoxic effects of drugs, both immune-mediated (allergic) and non-immune-mediated (pesudo-allergic) reactions.

A drug, before its release, is subjected to different phases of study: “in vitro” and “in vivo” studies on animals (pre-clinical experimentation) and so-called phase 1, phase 2 and phase 3 studies performed on humans (clinical experimentation).

In preclinical trials, to assess whether a drug can cause immunosuppression problems to the immune system, animals are immunized with specific antigens such as: KLH (Keyhole Limpet Hemocyanine), SRBC (Sheep Red Blood Cells), Tetanus toxoid, DNP (Dinitrophenol), TNP (Trinitrophenol).

Coated Surfaces for Immunotoxicity Test are suitable for measuring antibodies by ELISA assays. This test allows you to collect samples in series compared to other methods and minimize the quantities of reagents and antisera needed in the test.

General instructions for the use of microplates (on request Biomat can supply microplates for fluorescent and chemiluminescent assays).

Reagents supplied upon request.

Biomat’s Coated 96-Well Plates for Immunotoxicity

These are the available configurations of Immunotoxicity 96-Well Coated Plates products by Biomat.

Click on the configuration link to scroll down the page to the relative section.

Coated 96-Well Plates for Immunotoxicity ELISA Test main features

Biomat’s Coated 96-Well Plates for Immunotoxicity  have the following characteristics:

  • Ready to use
  • Manufactured under ISO:9001 guidelines
  • Post-coated (blocked) for low non specific binding and long-term stability
  • All lots are tested for uniformity and reproducibility
  • Certificate of Quality is released for every lot
  • For Research Use Only

Also, Coated Surface for Immunotoxicity ELISA Test Products ensures:

Uniformity

All the 96-Well Coated for Immunotoxicity Test  products show a CV% less than 5 when used as a sandwich of mouse IgG anti KHL (or anti DNP, anti TNP or anti Tetanus Toxoid)  in an ELISA format using goat anti mouse IgG-HRP as detector and TMB as substrate.

Storage and Stability

The microplates, if unopened, are stable at 2-8°C until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.

Coated Surfaces for Immunotoxicity ELISA Test Product specifications

KLH (Keyhole Limpet Hemocyanin)

The Californian giant keyhole limpet has a hemocyanin that has been recognized as a potent immunoactivator and therefore is widely used in research and clinical studies.

Keyhole limpet hemocyanin from Megathura crenulata is used as vaccine carrier protein. KLH is a copper containing respiratory protein, similar to hemoglobin in humans; it is a large protein that acts as the hapten carrier part of the vaccine component, and is so far thought to be non-toxic.

KLH is used as a highly immunogenic antigen in order to assess the immune competence of an organism and as a carrier of low molecular mass peptide and haptens, such as oligosaccharides, gangliosides or (glyco)peptides, designed to facilitate antibody production.

Examples of applications for KLH Coated 96-Well Plates:

  • assessing efficacy of vaccines, including dosage, adjuvantcy, route of immunization and timing
  • determination of immune status relative to controls
  • quantifying and standardizing vaccines batches and protocols

KLH Product Specifications

Coating

KLH is coated using 100 µl/well.

Binding capacity

Microplate was saturated with mouse IgG anti KLH at a concentration of 0.250 µg/ml (25 ng/well) in an ELISA format using goat anti mouse IgG-HRP as detector and TMB as substrate.

see KLH Coated Surface technical note

Result:

~ 0.250 µg /ml of mouse IgG anti KLH

DNP (Dinitrophenol)

Dinitrophenol is an hapten that can be attached to carrier proteins such as KLH (Keyhole Limpet Hemocyanin) or ovoalbumin; when this complex is injected into appropriate animal models, produces an immune response which is measured as changes in the levels of anti DNP IgM and IgG. Thanks to these changes, the researchers can assess the impact of pharmacologic or genetic manipulations on the studied immune system.

Examples of applications for DNP Coated 96-Well Plates:

  • used as biomarkers of immunotoxicity
  • assessing efficacy of vaccines, including dosage, adjuvantcy, route of immunization and timing
  • determination of immune status relative to controls

DNP Product Specifications

Coating

DNP-BSA is coated using 100 µl/well.

Binding capacity

Microplate was saturated with mouse IgG anti DNP at a concentration of 0.125 µg/ml (12.5 ng/well) in an ELISA format using goat anti mouse IgG-HRP as detector and TMB as substrate.

see DNP Coated Surface technical note

Result:

~ 0.125 µg /ml of mouse IgG anti DNP

TNP (Trinitrophenol)

Trinitrophenol is an hapten that can be attached to carrier proteins such as KLH (Keyhole Limpet Hemocyanin) or ovoalbumin; when this complex is injected into appropriate animal models, produces an immune response which is measured as changes in the levels of anti TNP IgM and IgG. Thanks to these changes, the researchers can assess the impact of pharmacologic or genetic manipulations on the studied immune system.

Examples of applications for TNP Coated 96-Well Plates:

  • used as biomarkers of immunotoxicity
  • assessing efficacy of vaccines, including dosage, adjuvantcy, route of immunization and timing
  • determination of immune status relative to controls

TNP Product Specifications

Coating

TNP-BSA is coated using 100 µl/well.

Binding capacity

Microplate was saturated with mouse IgG anti TNP at a concentration of 0.250 µg/ml (25 ng/well) in an ELISA format using goat anti mouse IgG-HRP as detector and TMB as substrate.

see TNP Coated Surface technical note

Result:

~ 0.250 µg /ml of mouse IgG anti TNP

Tetanus Toxoid

Tetanus, commonly called lockjaw, is a serious bacterial disease that affects muscles and nerves. It is characterized by muscle stiffness that usually involves the jaw and neck that then progresses to involve other parts of the body. This disease is caused by neurotoxin from deep wound infection with Clostridium tetani.

The use of this product can find application to set up assays where the level of anti-tetanus toxoid antibodies present in biological samples are measured spectrophotometrically, e.g. ELISA tests.

Examples of applications for Tetanus Toxoid Coated 96-Well Plates:

  • assessing efficacy of vaccines
  • quantifying and standardizing vaccines batches and protocols

Tetanus Toxoid Product Specifications

Coating

Tetanus Toxoid is coated using 100 µl/well.

Binding capacity

Microplate was saturated with mouse IgG anti Tetanus Toxoid at a concentration of 0.125 µg/ml (12.5 ng/well) in an ELISA format using goat anti mouse IgG-HRP as detector and TMB as substrate.

see Tetanus Toxoid Coated Surface technical note

Result:

~ 0.125 µg/ml of mouse IgG anti Tetanus Toxoid