Biotin coated surfaces offer a powerful instrument to carry out one of the most useful interactions in immunochemistry that involves the specificity binding of biotin to avidin, streptavidin or neutravidin. This binding shows a great constant affinity (10 – 15 M).
Biotin, or vitamin H (MW 244.31 Da), is a small naturally occurring cofactor that is present in every living cell in very minute amounts (usually less than 0.0001 %). The biotin molecule normally exists bound to proteins (such as pyruvate carboxylase) through its valeric acid carboxylic group by an amide bond to lysine side-chain amines.
The polystyrene optical features don’t change, allowing the modified surface to be used as a valid tool to carry out biological tests.
This surface shows its usefulness for these applications:
- interactions with avidin
- interactions with streptavidin
- interaction with neutravidin
96-well microplates, solid or with 12 removable 8-well strips.
Biotin (M.W. 244.31 Da) is coated using 200 µl/well. The strips are post-coated (blocked) for low non specific binding and long-term stability.
Microplates show a CV% less than 5 when streptavidin-HRP is used as detector and TMB as substrate in an ELISA format.
Storage and Stability
The microplates, if unopened, are stable refrigerated until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.