Biotin Coated Surface offers a powerful instrument to carry out one of the most useful interactions in immunochemistry that involves the specificity binding of biotin to avidin, streptavidin or neutravidin.

Biotin coated surfaces offer a powerful instrument to carry out one of the most useful interactions in immunochemistry that involves the specificity binding of biotin to avidin, streptavidin or neutravidin. This binding shows a great constant affinity (10– 15 M).

Biotin, or vitamin H (M.W. 244.31 Da), is a small naturally occurring cofactor that is present in every living cell in very minute amounts (usually less than 0.0001 %). The biotin molecule normally exists bound to proteins (such as pyruvate carboxylase) through its valeric acid carboxylic group by an amide bond to lysine side-chain amines.

The polystyrene optical features don’t change, allowing the modified surface to be used as a valid tool to carry out biological tests.

Examples of applications for Biotin Coated surface

  • interaction with avidin
  • interaction with streptavidin
  • interaction with neutravidin

Biomat Biotin Coated Products

Biotin Coated Surfaces main features

Biomat Biotin Coated products have the following characteristics:

  • Ready to Use
  • Manufactured under ISO:9001 guidelines
  • All lots are tested for uniformity and reproducibility
  • Certificate of Quality is released for every lot
  • For Research Use Only

Also, Biotin Coated Products ensures:

Uniformity

The Biotin Coated Products show a CV% less than 5 when streptavidin-HRP is used as detector and TMB as substrate in an ELISA format.

Storage and Stability

The Biotin Coated products, if unopened,  are stable at 2 – 8 °C until the expiration date printed on the label. If opened, store in a closed pouch with desiccant and use within the expiration date.

Biotin Coated Microplates Specifications

Coating

Biotin (M.W. 244.31 Da) is coated using 200 μl/well.

Binding specificity

The binding specificity of Biotin Coated 96-Well Plate was evaluated towards Streptavidin-HRP conjugate. Click on the following link to see the results.

Binding Specificity technical note