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Biotin Coated Surface offers a powerful instrument to carry out one of the most useful interactions in immunochemistry that involves the specificity binding of biotin to avidin, streptavidin or neutravidin.
Biotin coated surfaces offer a powerful instrument to carry out one of the most useful interactions in immunochemistry that involves the specificity binding of biotin to avidin, streptavidin or neutravidin. This binding shows a great constant affinity (10– 15 M).
Biotin, or vitamin H (M.W. 244.31 Da), is a small naturally occurring cofactor that is present in every living cell in very minute amounts (usually less than 0.0001 %). The biotin molecule normally exists bound to proteins (such as pyruvate carboxylase) through its valeric acid carboxylic group by an amide bond to lysine side-chain amines.
The polystyrene optical features don’t change, allowing the modified surface to be used as a valid tool to carry out biological tests.
Examples of applications for Biotin Coated surface
- interaction with avidin
- interaction with streptavidin
- interaction with neutravidin
Biomat Biotin Coated Products
Biotin Coated Surfaces main features
Biomat Biotin Coated products have the following characteristics:
- Ready to Use
- Manufactured under ISO:9001 guidelines
- All lots are tested for uniformity and reproducibility
- Certificate of Quality is released for every lot
- For Research Use Only
Also, Biotin Coated Products ensures:
Uniformity
The Biotin Coated Products show a CV% less than 5 when streptavidin-HRP is used as detector and TMB as substrate in an ELISA format.
Storage and Stability
The Biotin Coated products, if unopened, are stable at 2 – 8 °C until the expiration date printed on the label. If opened, store in a closed pouch with desiccant and use within the expiration date.
Biotin Coated Microplates Specifications
Coating
Biotin (M.W. 244.31 Da) is coated using 200 μl/well.
Binding specificity
The binding specificity of Biotin Coated 96-Well Plate was evaluated towards Streptavidin-HRP conjugate. Click on the following link to see the results.
