Biomat’s Secondary Antibodies Coated Surface, available with Goat Anti-Mouse IgG and Goat Anti-Rabbit IgG, is specifically designed for antibody-binding assays where direct adsorption to polystyrene can compromise antibody integrity. This surface is ideal for cases where primary antibodies are limited in quantity or prone to denaturation, ensuring optimal assay sensitivity and consistency.
When and Why to Use a Secondary Antibody Coated Surface
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Prevents antibody denaturation caused by direct adsorption onto polystyrene
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Ensures higher binding efficiency for diluted antibody solutions, especially mouse and rabbit IgG
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Fc-specific binding offers greater selectivity than Protein A or Protein G plates
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Ideal for indirect ELISA, sandwich ELISA, competitive assays, isotyping, and hybridoma screening
Unlike Protein A or G-coated surfaces, Biomat’s secondary antibody-coated surfaces bind exclusively to the IgG class of the selected species, offering enhanced specificity and compatibility with downstream detection protocols.
Biomat’s Secondary Antibodies Coated 96-Well Plates product
These are the available configurations of Secondary Antibodies 96-Well Coated Plates products by Biomat.
Click on the configuration link to scroll down the page to the relative section.
Explore our Full Range of 96-Well Plates
Key Features of Biomat’s Secondary Antibody Coated Surface
Biomat Coated Plates with Secondary Antibodies are suitable with ELISA Assay Technology due to these features:
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Fc-specific surface binding for mouse and rabbit IgG
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Ready-to-use 96-well format
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Made of pure polystyrene with low fluorescence background
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Post-coated (blocked) to minimize non-specific binding
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Alphanumeric coding for accurate well identification
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SBS-standard compliant, automation-friendly design
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Manufactured under ISO 9001:2015 quality standards
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Lot-specific Certificate of Quality included
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For Research Use Only
Surface Performance & Uniformity
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Uniform coating ensures coefficient of variation (CV%) below 5%
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Compatible with ELISA workflows using anti-IgG-HRP conjugates and TMB substrate
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Reliable detection of low-abundance antibodies across multiple applications
Storage and Stability
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Store unopened plates at 2–8°C until expiration date (printed on the label)
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After opening, keep in sealed pouch with desiccant; use before expiration
Secondary Antibodies Coated Surfaces Product specifications
Goat Anti Mouse IgG Fcγ (Subclasses 1+2a+2b+3)
Affinity purified goat anti mouse IgG specifically binds the Fc region of mouse immunoglobulin subclasses 1, 2a, 2b and 3, with minimal cross-reaction to human, bovine and rabbit serum proteins.
These plates may be used as solid support for most sandwich ELISAs utilizing a mouse IgG capture and a non mouse IgG detection antibody. Other applications include competitive ELISA, IgG isotyping and hybridoma screening/selection.
Examples of applications for Goat Anti Mouse IgG Fcγ Coated 96-Well Plates:
- prevent antibody denaturation as a result of direct adsorption to polystyrene
- unlike Protein A or G plates, these plates bind only to target IgG species
- these plates show a higher antibody-binding capacity than direct adsorption onto polystyrene when using diluted mouse IgG solutions
Goat Anti Mouse IgG Fcγ Coated 96-Well Plates Product Specifications
Coating
Affinity purified goat anti mouse IgG Fcγ (subclasses 1+2a+2b+3) is coated using 100 μl/well.
Binding capacity
Microplate was saturated with mouse IgG at a concentration of 1.0 μg/ml (100 ng/well) in an ELISA format using goat anti mouse IgG (H+L)-HRP as detector and TMB as substrate.
see Binding Capacity technical note
Sensitivity
Mouse IgG was detected at a concentration significantly above background in an ELISA format using goat anti mouse IgG (H+L)-HRP as detector and TMB as substrate.
see Sensitivity technical note
Goat Anti Rabbit IgG Fc
Affinity purified goat anti rabbit IgG specifically binds the Fc region of rabbit immunoglobulins , with minimal crossreaction to human serum proteins.
These plates may be used as solid support for most sandwich ELISA utilizing a mouse IgG capture and a non mouse IgG detection antibody. Other applications include competitive ELISA, IgG isotyping and hybridoma screening/selection.
Examples of applications for Goat Anti Rabbit IgG Fc Coated 96-Well Plates:
- prevent antibody denaturation as a result of direct adsorption to polystyrene
- unlike Protein A or G plates, these plates bind only to target IgG species
- these plates show a higher antibody-binding capacity than direct adsorption onto polystyrene when using diluted rabbit solutions
Goat Anti Rabbit IgG Fc Coated 96-Well Plates Product Specifications
Coating
Affinity purified Goat anti rabbit IgG Fc is coated using 100 μl/well.
Binding capacity
Microplate was saturated with rabbit IgG at a concentration of 1.0 μg/ml (100 ng/well) in an ELISA format using goat anti rabbit IgG (H+L)-HRP as detector and TMB as substrate.
see Binding Capacity technical note
Sensitivity
Rabbit IgG was detected at a concentration significantly above background in an ELISA format using goat anti rabbit IgG ( H+L )-HRP as detector and TMB as substrate.
see Sensitivity technical note
