Secondary Antibodies Coated Surface, both Goat Anti Mouse IgG and Goat Anti Rabbit IgG, is ideal for binding assays where available antibodies are in low quantities or they denature and become inactive upon direct adsorption to polystyrene plates.

These surfaces are ideal for binding assays when available antibodies are in low quantities or they denature and become inactive upon direct adsorption to polystyrene plates.

Features of secondary antibodies coated plates:

  • prevent antibody denaturation as a result of direct adsorption to polystyrene
  • unlike Protein A or G plates, these plates bind only to target IgG species
  • these plates show a higher antibody-binding capacity than direct adsorption onto polystyrene when using diluted rabbit solutions

Available configurations

Biomat’s Secondary Antibodies surfaces are available on the following Biomat’s products:

Storage and Stability

The microplates, if unopened, are stable refrigerated until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.

 

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GOAT ANTI RABBIT IgG Fc

Affinity purified goat anti rabbit IgG specifically binds the Fc region of rabbit immunoglobulins , with minimal crossreaction to human serum proteins.

Product specifications

Coating

Affinity purified Goat anti rabbit IgG Fc is coated using 100 μl/well. The strips are post-coated (blocked) for low non specific binding and long-term stability.

Binding capacity

Microplate was saturated with rabbit IgG at a concentration of 1.0 μg/ml (100 ng/well) in an ELISA format using goat anti rabbit IgG (H+L)-HRP as detector and TMB as substrate.

see Binding Capacity technical note 

Result:

~ 0.625 pmol/well of rabbit IgG

Sensitivity

Rabbit IgG was detected at a concentration significantly above background in an ELISA format using goat anti rabbit IgG ( H+L )-HRP as detector and TMB as substrate.

see Sensitivity technical note

Result:

~ 0.01 μg/ml of rabbit IgG

Uniformity

Microplates show a CV% less than 5 when used as a catcher of rabbit IgG in an ELISA format using goat anti rabbit IgG ( H+L )-HRP as detector and TMB as substrate.

GOAT ANTI MOUSE IgG Fcγ (Subclasses 1+2a+2b+3)

Affinity purified goat anti mouse IgG specifically binds the Fc region of mouse immunoglobulin subclasses 1, 2a, 2b and 3, with minimal cross-reaction to human, bovine and rabbit serum proteins.
These plates may be used as solid support for most sandwich ELISAs utilizing a mouse IgG capture and a non mouse IgG detection antibody. Other applications include competitive ELISA, IgG isotyping and hybridoma screening/selection.

Product specifications

Coating

Affinity purified goat anti mouse IgG Fcγ (subclasses 1+2a+2b+3) is coated using 100 μl/well. The strips are postcoated (blocked) for low non specific binding and long-term stability.

Binding capacity

Microplate was saturated with mouse IgG at a concentration of 1.0 μg/ml (100 ng/well) in an ELISA format using goat anti mouse IgG (H+L)-HRP as detector and TMB as substrate.

see Binding Capacity technical note

Result:

~ 0.625 pmol/well of mouse IgG

Sensitivity

Mouse IgG was detected at a concentration significantly above background in an ELISA format using goat anti mouse IgG (H+L)-HRP as detector and TMB as substrate.

see Sensitivity technical note

Result:

~ 0.01 μg/ml of mouse IgG

Uniformity

Microplates show a CV% less than 5 when used as a catcher of mouse IgG in an ELISA format using goat anti mouse IgG (H+L)-HRP as detector and TMB as substrate.