Secondary Antibodies Coated Surface, both Goat Anti Mouse IgG and Goat Anti Rabbit IgG, is ideal for binding assays where available antibodies are in low quantities or they denature and become inactive upon direct adsorption to polystyrene plates.

The surface of Secondary Antibodies Coated 96-Well Plates is ideal for binding assays where available antibodies are in low quantities or they denature and become inactive upon direct adsorption to polystyrene plates.

Features of Secondary Antibodies Coated 96 Well Plate:

  • prevent antibody denaturation as a result of direct adsorption to polystyrene
  • unlike Protein A or G plates, these plates bind only to target IgG species
  • these plates show a higher antibody-binding capacity than direct adsorption onto polystyrene when using diluted mouse solutions
  • used for direct, indirect, competitive or sandwich ELISA assays

Biomat’s Secondary Antibodies Coated 96-Well Plates product

These are the available configurations of Secondary Antibodies 96-Well Coated Plates products by Biomat.

Click on the configuration link to scroll down the page to the relative section.

Other 96-Well Plates

Secondary Antibodies Coated Surface main features

Biomat Coated Plates with Secondary Antibodies are suitable with ELISA Assay Technology due to these features:

  • Ready to use
  • Manufactured under ISO:9001 guidelines
  • Made in pure polystyrene with low fluorescence
  • Post-coated (blocked) for low non specific binding and long-term stability
  • Alphanumeric coding for easy well recognition
  • Microplates comply with SBS standards and the design assures a good performance in automatic processing plant
  • All lots are tested for uniformity and reproducibility
  • Certificate of Quality is released for every lot
  • for Research Use Only

Also, Secondary Antibodies Coated 96-Well Plates ensure:

Uniformity

The Secondary Antibodies Coated products show a CV% less than 5 when used as a catcher of IgG in an ELISA format using anti IgG-HRP as detector and TMB as substrate.

Storage and Stability

The microplates, if unopened, are stable at 2-8°C until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.

Secondary Antibodies Coated Surfaces Product specifications

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Goat Anti Mouse IgG Fcγ (Subclasses 1+2a+2b+3)

Goat Anti Rabbit IgG Fc

 

Goat Anti Mouse IgG Fcγ (Subclasses 1+2a+2b+3)

Affinity purified goat anti mouse IgG specifically binds the Fc region of mouse immunoglobulin subclasses 1, 2a, 2b and 3, with minimal cross-reaction to human, bovine and rabbit serum proteins.

These plates may be used as solid support for most sandwich ELISAs utilizing a mouse IgG capture and a non mouse IgG detection antibody. Other applications include competitive ELISA, IgG isotyping and hybridoma screening/selection.

Examples of applications for Goat Anti Mouse IgG Fcγ Coated 96-Well Plates:

  • prevent antibody denaturation as a result of direct adsorption to polystyrene
  • unlike Protein A or G plates, these plates bind only to target IgG species
  • these plates show a higher antibody-binding capacity than direct adsorption onto polystyrene when using diluted mouse IgG solutions

Goat Anti Mouse IgG Fcγ Coated 96-Well Plates Product Specifications

Coating

Affinity purified goat anti mouse IgG Fcγ (subclasses 1+2a+2b+3) is coated using 100 μl/well.

Binding capacity

Microplate was saturated with mouse IgG at a concentration of 1.0 μg/ml (100 ng/well) in an ELISA format using goat anti mouse IgG (H+L)-HRP as detector and TMB as substrate.

see Binding Capacity technical note

Result:

~ 0.625 pmol/well of mouse IgG

Sensitivity

Mouse IgG was detected at a concentration significantly above background in an ELISA format using goat anti mouse IgG (H+L)-HRP as detector and TMB as substrate.

see Sensitivity technical note

Result:

~ 0.01 μg/ml of mouse IgG

Goat Anti Rabbit IgG Fc

Affinity purified goat anti rabbit IgG specifically binds the Fc region of rabbit immunoglobulins , with minimal crossreaction to human serum proteins.

These plates may be used as solid support for most sandwich ELISA utilizing a mouse IgG capture and a non mouse IgG detection antibody. Other applications include competitive ELISA, IgG isotyping and hybridoma screening/selection.

Examples of applications for Goat Anti Rabbit IgG Fc Coated 96-Well Plates:

  • prevent antibody denaturation as a result of direct adsorption to polystyrene
  • unlike Protein A or G plates, these plates bind only to target IgG species
  • these plates show a higher antibody-binding capacity than direct adsorption onto polystyrene when using diluted rabbit solutions

Goat Anti Rabbit IgG Fc Coated 96-Well Plates Product Specifications

Coating

Affinity purified Goat anti rabbit IgG Fc is coated using 100 μl/well.

Binding capacity

Microplate was saturated with rabbit IgG at a concentration of 1.0 μg/ml (100 ng/well) in an ELISA format using goat anti rabbit IgG (H+L)-HRP as detector and TMB as substrate.

see Binding Capacity technical note

Result:

~ 0.625 pmol/well of rabbit IgG

Sensitivity

Rabbit IgG was detected at a concentration significantly above background in an ELISA format using goat anti rabbit IgG ( H+L )-HRP as detector and TMB as substrate.

see Sensitivity technical note

Result:

~ 0.01 μg/ml of rabbit IgG