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Coated PCR products

Molecular Grade Plates Market (2021)

Source: Persistence Market Research

Quote: “For instance, Biomat, a worldwide manufacturer of plates and tubes for ELISA and PCR assays tests, with leading expertise in surface modification, has provided Biomat PCR-coated tubes and plates. These products offer a viable support for the development of COVID-19 PCR tests kits for life science manufacturers and researchers.”

Profiling surface proteins on individual exosomes using a proximity barcoding assay (2019)

Authors: Di Wu, Junhong Yan, Xia Shen, Yu Sun, Måns Thulin, Yanling Cai, Lotta Wik, Qiujin Shen, Johan Oelrich, Xiaoyan Qian, K. Louise Dubois, K. Göran Ronquist, Mats Nilsson, Ulf Landegren & Masood Kamali-Moghaddam.

Quote: “Probing and capturing exosomes. For each reaction, 50 ng biotinylated CTB (C-34779, Thermo Scientific) was diluted in 25 μl PBS and incubated overnight in STV-coated PCR tubes (PCR0STF-SA5/100, Biomat). The wells in the plates were then washed twice with 100 μl washing buffer. Exosomes from each sample source or cell line was mixed with all antibody conjugates (20 nM for each), directed against surface proteins, in PBA buffer (PBS, 0.05% Tween 20, 5 mM EDTA, 1 mg/ ml salmon sperm DNA (15632011, ThermoFisher Scientific) and 1% BSA) at 4 °C overnight. After incubation, 1 μl of the exosome-antibody conjugate complexes were diluted in 25 μl PBA buffer, and 20 μl was incubated in wells coated with biotinylated CTB for 15 min at RT. For replicate measurements, 2 μl of exosomes were diluted in 50 µl PBA buffer, and 20 μl were added into two separated wells.”

Patent: Surface-based tagmentation (2018)

Inventors: Gormley Niall Anthony (Saffron Walden, Essex, GB), Ioannou Avgousta (Saffron Walden, Essex, GB), Jackson Rosamond (Saffron Walden, Essex, GB),  Morrell Natalie (Saffron Walden, Essex, GB)

Quote: “… on library yield, two sets of customized tubes that have higher concentrations of streptavidin coatings were obtained from Biomat (Rovereto, Italy) … from the filter paper sheet using an automated or manual hole-punch and placed into a sample tube (eg, PCR tubes) …”

Coated 96 Well Plates

Quantitative fluorescent nanoparticle tracking analysis and nano-flow cytometry enable advanced characterization of single extracellular vesicles (2024)

Authors: Danilo Mladenović, Joseph Brealey, Ben Peacock, Nataša Zarovni
Quote: “Yellow-green FS beads and AF488-labeled antibodies were serially diluted in PBS and loaded in a black polystyrene 96-well plate with no binding capacity (Biomat, Italy). “

Fish Probiotics: Cell Surface Properties of Fish Intestinal Lactobacilli and Escherichia coli (2023)

Authors: Susanna Mirzabekyan, Natalya Harutyunyan, Անահիտ Մանվելյան, Lilit Malkhasyan, Մարինե Բալայան, Shakhlo Miralimova, Michael L. Chikindas, V. A. Chistyakov, Astghik Pepoyan

Quote: “Specifically, 200µ L of bacterial suspensions (OD600 = 0.5) incubated overnight, was transferred into a polystyrene 96-well plate (Biomat, Ala, Italy)and incubated for 48 h at 37◦C.”

A Quantitative ELISA to Detect Anti-SARS-CoV-2 Spike IgG Antibodies in Infected Patients and Vaccinated Individuals (2022)

Authors: Ji Luo, Jennifer Klett, Jörg Gabert, Thomas Lipp, Julia Karbach, Elke Jäger, Stephan Borte,Ralf Hoffmann, and Sanja Milkovska-Stamenova.
Quote: “An indirect ELISA for anti-SARS-CoV-2 spike IgG antibodies was established using a recombinant trimeric spike protein. To optimize the coating conditions, four microtiter plate types including maxisorp (Nunc), which consists of a modified, highly charged polystyrene surface with high affinity to molecules with polar or hydrophilic groups, medium binding (Brand; Greiner), and high binding (Biomat) were compared using a positive serum pool and the negative serum pool (Supplementary Figure S1A). “

Optimization of Tris/EDTA/Sucrose (TES) periplasmic extraction for the recovery of functional scFv antibodies (2020)

Authors: Elham Ghamghami, Marjan Abri Aghdam, Mohammad Reza Tohidkia, Asadollah Ahmadikhah, Morteza Khanmohammadi, Tayebeh Mehdipour, Ahad Mokhtarzadeh, and Behzad Baradaran

Quote: “High-binding 96-well ELISA plates (Biomat) were coated with biotinylated G17-Gly peptide (pEGPWLEEEE-K-s–s-biotin, where pE means pyroglutamic acid) indirectly through the biotin-streptavidin system (Tohidkia et al. ). “

Improved Soluble ScFv ELISA Screening Approach for Antibody Discovery Using Phage Display Technology (2017)

New immunoassays for total, IgA and IgM antibodies against hepatitis E virus: Prevalence in Italian blood donors and patients with chronic liver or kidney diseases (2016)

Authors: Gabriele Ricco, Ferruccio Bonino, Maria Lanza, Fabrizio Scatena, Carlo M.Alfieri, Piergiorgio Messa, Edoardo Marchisi, Giovanni Mascolo, Luisa Romanò, Cristina Galli, Tian ChengLi, Takaji Wakita, Tatsuo Miyamura, Maurizia R.Brunetto

Quote: “… 2.2.2. Total anti-HEV assay. Polystirene micro-plates (BiomatItaly) were coated with purified HEV-LPs dissolved in PBS and incubated overnight at room temperature. After washing, micro-plates were post-coated with 1% BSA in PBS and then dried …”

Syphilis Diagnosis Using an Advance Concept for NonTreponemal Test Development (2015)

Authors: Mayur R. Shukla1 and Himanshu C. Mody

Quote: “… This antigen was attached directly to microwell plate with primary amine group on it. Such type of plate can be purchased commercially from different suppliers like Biomat (Italy)  …”

β-Actin in the Signaling of Transmembrane TNF-α-Mediated Cytotoxicity (10 April 2014)

Authors: H Chen, Y Leng, Z Li – The TNF Superfamily, 2014 – Springer

Quote: “… NIH3T3 cell line was stably transfected with an empty retrovirus plasmid pLXSN as a control, or pLW-TNF-α, the plasmid inserted with human wtTNF-α cDNA at the HpaI and XhoI cloning site, in our laboratory [19] (see Note 2 ). 4. 96-Well microtiter plates (BiomatItaly). 5 …”

Determination of a new antibacterial peptide S-thanatin in rat plasma by an indirected-ELISA (20011)

Authors:Guoqiu Wu, Shenglan Wang, Xiyong Wang, Xiaofang Li, Xuepeng Deng, Zilong Shen, Tao Xi

Quotes: “… Then 96well flat-bottom microtiter plates (Biomat, Rovereto, Italy) were filled with…”

Development of an ELISA reverse-based assay to assess the presence of mycotoxins in cereal flour (2011)

Authors: Silvia Folloni, Gianni Bellocchi, Dafni-Maria Kagkli, Susana Pastor-Benito, Margarita Aguilera, Alessandra Mazzeo, Maddalena Querci, Guy Van den Eede, Monica Ermolli

Quote: “… 1) were produced by Biomat snc (Rovereto, Italy) while low binding microplates were purchased …
Assay. One hundred microliters of standards or samples were distributed into microplate wells
and 200 µL of horseradish peroxidase conjugated mycotoxins were added (final …”

The level of connective tissue growth factor in sera of patients with hepatitis B virus strongly correlates with stage of hepatic fibrosis (2010)

Authors: Wu Guo-Qiu, Liu Nai-Feng, Fan Xiao-Bo, Li Linxian, Zhang Chen, Gou Lixia, and Liu Zhao

Quote:“… Then 96well flat-bottom microtiter plates (Biomat, Rovereto, Italy) were filled with 100 μL/well
of a solution containing 10 μg/mL of purified recombinant CTGF, and were coated overnight at 4°C….”

Development and validation of a ζ-globin-specific ELISA for carrier screening of the (−− SEA) α thalassaemia deletion (2009)

Authors: L Tang, P Zhu, W J Zhou, J Zheng, Y Q Zhou, N Fu, X M Xu

Quote: “… Other ELISA conditions had also been optimised in the following manner: the microplate was coated with 100 μl/well 4D11 at 4°C … with those of the relatively high-cost products, including Immuno Plates (Nunc, Rochester, New York, USA), HTS microplates (Biomat Roverto, Italy) …”

The Influence of Membrane Lipids in Staphylococcus aureus Gamma-Hemolysins Pore Formation (2008)

Authors: C. Potrich, H. Bastiani, D. A. Colin, S. Huck, G. Prévost, M. Dalla Serra

Quote: “… was measured with a fluorescence microplate reader (Fluostar BMG). Samples were excited through a narrow-band interference filter centered at 485 nm, and the fluorescence was detected after a second filter at 538 nm. White plastic 96-well microplates (Biomat, Rovereto, Italy …” 

Development of a multiplex immunoassay based on the ELISA Reverse m&d for the simultaneous detection of enzootic bovine leukosis (EBL), brucellosis (B) and paratuberculosis (JD) in bovine bulk milk samples (2008)

Authors: Monica Ermolli, Silvia Folloni, Adelina Prospero, Gianni Bellocchi, Maddalena Querci, Guido Petracca, Massimo Biagetti, Alessandra Mazzeo and Guy Van den Eede – Joint Research Center

Quote:”…  Materials and methods The ER device is constituted of an immunosorbent polystyrene high-binding (HB10) rod with 4 protruding ogives (Biomat, Italy) (Fig.1).

Development of an innovative immunoassay for CP4EPSPS and Cry1AB genetically modified protein detection and quantification (2006)

Authors: M. Ermolli, A. Prospero, B. Balla, M. Querci, A. Mazzeo & G. Van Den Eede

Quote: “… Plastic support Prototypes of polystyrene-activated devices HB10 were produced from Biomat snc (Rovereto, Trento, Italy)…”