Protein A/G Coated Plates are designed to provide alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA.
The Protein A/G surface of Protein A/G Coated Plates is a surface that binds to most IgG species (in particular binds to all subclasses of mouse IgG excluding mouse IgA, IgM or serum albumin and to all subclasses human IgG),IgA, IgE, IgM and IgD.
Protein A/G includes four Fc binding domains from Protein A and two from Protein G making it a versatile tool. The binding dependency to pH of Protein A/G is lower than Protein A but has the additive properties of Protein A and G together.
Examples of applications for Protein A/G Coated Plates are:
specific and sterically oriented bond of antibodies
purification and detection of mouse monoclonal IgG antibodies and for purification of macaque IgG
highest specificity and capacity
retains antibody activity and orients antibody for maximum binding
generally not suitable for sandwich ELISA assays
Biomat Protein A/G Coated 96-Well Plates are made in Transparent, White and BlackPolystyrene and can be used for quantitative and qualitative immunoassays like ELISA, Chemiluminescent (CLIA) , Luminescence (LIA) and Fluorescence (FIA) assays.
The Plates are pre-blocked in order to minimize any non-specific binding and to ensure long-term stability.