The neutravidin coated surfaces offer a powerful and universal instrument for binding biotinylated molecules minimizing non-specific interactions.

Neutravidin is a deglycosylated avidin (M.W. 60 kDa) that contains four identical subunits biotin-binding with very high affinity for biotin (Ka= 10-15 M).

It has an isoelectric point near-neutral (pI=6.3) and the lowest non specific binding properties among the known biotin binding proteins.

Compared to the streptavidin, neutravidin in its primary structure does not contain the RYD sequence (Arg-Tyr-Asp): the lack of such a sequence totally eliminates the possibility to interact with the RGD sequence (Arg-Gly-Asp) present in the membrane receptors of a large variety of cells.

This makes it very useful in applications where it is important to avoid the non-specific interaction with the cell surfaces.

Neutravidin coated surface has similar binding capacity of streptavidin coated surface for biotin and biotinylated molecules.

The neutravidin coated surfaces offer a powerful and universal instrument for binding biotinylated molecules minimizing non-specific interactions.

Product specifications

Available configurations

96-well microplates, solid or with 12 removable 8-well strips.

Coating

Neutravidin is coated using 100 µl/well. The strips are post-coated (blocked) for low non specific binding and long-term stability.

Binding capacity for small molecules (e.g. d-biotin)

The Neutravidin coated wells were incubated with biotin solutions, in an ELISA format, using biotinylated peroxidase as detector and TMB as substrate.

Result:

~ 5 pMol d-biotin/well

Binding capacity for big molecules (e.g. HIgG)

The Neutravidin coated wells were incubated with biotinylated HIgG in an ELISA format using HIgG-HRP as a detector and TMB as substrate.

see Binding Capacity technical note

Result:

~ 100 ng/ml biotinylated IgG

Uniformity

Microplates show a CV% less than 5 in an ELISA format, using biotin-HRP as detector and TMB as substrate.

Storage and Stability

The microplates, if unopened, are stable refrigerated until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.
see Stability Test technical note