Protein A/G Coated Surface provides an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plates-based assay techniques.
Protein A/G includes four Fc binding domains from Protein A and two from Protein G making it a versatile tool. The binding dependency to pH of Protein A/G is lower than Protein A but has the additive properties of Protein A and G together.
The Protein A/G binds to human IgG, IgA, IgM; it binds to all subclasses of mouse IgG excluding mouse IgA, IgM and serum albumin (see Table 1) . When coated onto microplates, the Protein A/G can securely be used in purification and detection of mouse monoclonal IgG antibodies with no interference from IgA, IgM and serum albumin.
Example of applications:
- specific and sterically oriented bond of antibodies
- highest specificity and capacity
- retains antibody activity and orients antibody for maximum binding
- generally not suitable for sandwich ELISA assays
Biomat ‘s Protein A/G Products
Biomat ‘s Protein A/G surfaces are available on the following Biomat’s products:
Protein A/G Products Main Features
Uniformity
The coated products show a CV% less than 10.
Storage and Stability
The coated products, if unopened, are stable at 2-8°C until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.
Protein A/G Plates Specifications
Coating
Recombinant Protein A/G (M.W. 50.4 kDa) is a fusion protein between Protein A and Protein G. The Protein A portion is from Staphylococcus aureus segments E, D, A, B and C and the Protein G portion is from Streptococcus sp. segments C1 and C3, expressed in E. coli. Protein A/G is coated using 200 µl/well. The strips are post-coated (blocked) for low non specific binding and long-term stability.
Binding capacity
Microplate was saturated with biotinylated human IgG at a concentration of 0.4-0.5 µg/ml (400-500 ng/well) in an ELISA format using Streptavidin-HRP diluted mixed with Streptavidin as detector and TMB as substrate.
2.66 - 3.33 pMol IgG/well (100 μl volume)
Protein A/G PCR 8 Strip Tubes and PCR Plates Specifications
Coating
Recombinant Protein A/G (M.W. 50.4 kDa) is a fusion protein between Protein A and Protein G. The Protein A portion is from Staphylococcus aureus segments E, D, A, B and C and the Protein G portion is from Streptococcus sp. segments C1 and C3, expressed in E. coli. Protein A/G is coated using 100 µl/tube. The PCR 8 strip tubes and plates are post-coated (blocked) for low non specific binding and long-term stability.
