Protein A/G Coated Surface provides an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plates-based assay techniques.

Protein A/G Coated Surface is a surface that binds to most IgG species (in particular binds to all subclasses of mouse IgG excluding mouse IgA, IgM or serum albumin and to all subclasses human IgG), IgA, IgE, IgM and IgD.

Protein A/G includes four Fc binding domains from Protein A and two from Protein G making it a versatile tool. The binding dependency to pH of Protein A/G is lower than Protein A but has the additive properties of Protein A and G together.

Protein A/G binds to human IgG, IgA, IgM; it binds to all subclasses of mouse IgG excluding mouse IgA, IgM and serum albumin (see Table 1). When coated onto microplates, the Protein A/G can securely be used in purification and detection of mouse monoclonal IgG antibodies with no interference from IgA, IgM and serum albumin.

Examples of applications for Protein A/G Coated surface

  • specific and sterically oriented bond of antibodies
  • highest specificity and capacity
  • retains antibody activity and orients antibody for maximum binding
  • generally not suitable for sandwich ELISA assays

Biomat Protein A/G Coated Products

96-Well Plates

Other 96-Well Plates

PCR Products

Other PCR Products

Protein A/G Coated Surfaces main features

Biomat Protein A/G Coated products have the following characteristics:

  • Ready to Use
  • Manufactured under ISO:9001 guidelines
  • All lots are tested for uniformity and reproducibility
  • Certificate of Quality is released for every lot
  • For Research Use Only

Also, Protein A/G  Coated Products ensures:

Uniformity

The Protein A/G Coated Products show a CV% less than 10 when used as a catcher of biotinylated human IgG in an ELISA format using streptavidin-HRP as detector and TMB as substrate.

Storage and Stability

The Protein A/G Coated products, if unopened,  are stable at 2 – 8 °C until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.

Protein A/G Coated Microplates Specifications

Coating

Recombinant Protein A/G (M.W. 50.4 kDa) is a fusion protein between Protein A and Protein G. The Protein A portion is from Staphylococcus aureus segments E, D, A, B and C and the Protein G portion is from Streptococcus sp. segments C1 and C3, expressed in E. coli. Protein A/G is coated using 200 µl/well.

Binding capacity

Microplate was saturated with biotinylated human IgG at a concentration of 0.4-0.5 µg/ml (400-500 ng/well) in an ELISA format using Streptavidin-HRP diluted mixed with Streptavidin as detector and TMB as substrate.

see Binding Capacity technical note

Results:

2.66 - 3.33 pMol IgG/well (100 μl volume)

Protein A/G Coated PCR 8 Strip Tubes and PCR Plates Specifications

Coating

Recombinant Protein A/G (M.W. 50.4 kDa) is a fusion protein between Protein A and Protein G. The Protein A portion is from Staphylococcus aureus segments E, D, A, B and C and the Protein G portion is from Streptococcus sp. segments C1 and C3, expressed in E. coli.  Protein A/G is coated using 100 µl/tube.