Protein G Coated Surface offers a powerful and sensitive instrument designed for the capture of IgG applied directly or as antibody/antigen complex.
Protein G Coated Surface provides an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plates-based assay techniques.
Protein G (Recombinant Protein G – M.W. 26.1 kDa) from Streptococcus sp., expressed in E. coli, specifically binds the Fc region of immunoglobulins of many mammalian species (see Table 1) , with an orientation that allows the F(ab)2 binding sites to be freely available for efficient binding to epitope. When coated onto microplates, the Protein G can securely capture IgG applied directly or as antigen/antibody complexes.
Examples of applications for Protein G Coated surface
- specific and sterically oriented bond of IgG
- separation of IgG from other immunoglobulins
- separation of antigen-antibodies complexes
- isolation and analysis of fusion proteins
Biomat Protein G Coated Products
Protein G Coated Surfaces main features
Biomat Protein G Coated products have the following characteristics:
- Ready to Use
- Manufactured under ISO:9001 guidelines
- All lots are tested for uniformity and reproducibility
- Certificate of Quality is released for every lot
- For Research Use Only
Also, Protein G Coated Products ensures:
The Protein G Coated Products show a CV% less than 10 when used as a catcher of biotinylated human IgG in an ELISA format using streptavidin-HRP as detector and TMB as substrate.
Storage and Stability
The Protein G Coated products, if unopened, are stable at 2 – 8 °C until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.
Protein G Coated Microplates Specifications
Recombinant Protein G ( M.W. 26.1 kDa ), from Streptococcus sp., expressed in E. coli, is coated using 200 µl/well.
Microplate was saturated with biotinylated human IgG at a concentration of 0.4-0.5 µg/ml (400-500 ng/well) in an ELISA format using Streptavidin-HRP diluted mixed with Streptavidin as detector and TMB as substrate.
2.66 - 3.33 pMol IgG/well (100 μl volume)
Biotinylated human IgG was detected at a concentration significantly above background in an ELISA format using streptavidin-HRP as detector and TMB as substrate.
0.056 ng/well of human IgG
Protein G Coated PCR 8 Strip Tubes and PCR Plates Specifications
Recombinant Protein G ( M.W. 26.1 kDa ), from Streptococcus sp., expressed in E. coli, is coated using 100 µl/tube.