Protein G (Recombinant Protein G – M.W. 26.1 kDa) from Streptococcus sp., expressed in E. coli, specifically binds the Fc region of immunoglobulins of many mammalian species (see Table 1) , with an orientation that allows the F(ab)2 binding sites to be freely available for efficient binding to epitope. When coated onto microplates, the Protein G can securely capture IgG applied directly or as antigen/antibody complexes.
Example of applications:
- specific and sterically oriented bond of IgG
- separation of IgG from other immunoglobulins
- separation of antigen-antibodies complexes
- isolation and analysis of fusion proteins
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The coated products show a CV% less than 10 when used as a catcher of biotinylated human IgG in an ELISA format using streptavidin-HRP as detector and TMB as substrate.
Storage and Stability
The coated products, if unopened, are stable at 2-8°C until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.
Product specifications for microplates
Recombinant Protein G (M.W. 26.1 kDa) ,from Streptococcus sp., expressed in E.coli, is coated using 200 μl/well. The strips are post-coated (blocked) for low non specific binding and long-term stability.
Microplate was saturated with biotinylated human IgG at a concentration of 0.4-0.5 µg/ml (400-500 ng/well) in an ELISA format using Streptavidin-HRP diluted mixed with Streptavidin as detector and TMB as substrate.
2.66 - 3.33 pMol IgG/well (100 μl volume)
Biotinylated human IgG was detected at a concentration significantly above background in an ELISA format using streptavidin-HRP as detector and TMB as substrate.
0.056 ng/well of human IgG
Product specifications for PCR 8 strip tubes and plates
Recombinant Protein G (M.W. 26.1 kDa) ,from Streptococcus sp., expressed in E.coli, is coated using 100 µl/tube. The PCR 8 strip tubes and plates are post-coated (blocked) for low non specific binding and long-term stability.