Maleimide coated surfaces offer a powerful instrument for binding biomolecules containing free sulfydryl groups (e.g. peptides that contain a terminal cysteine or thiol containing haptens ) , or reducibile disulfide bonds that are difficult to coat onto polystyrene plates. These coated plates are a very useful tool for assays requiring site-directed orientation of particular biomolecules especially during antibody production.
At pH 6.5-7.5 maleimide reacts with free sulfydyl groups to yeld stable bonds, while the reaction with amine becomes significant at pH > 7.5.
If sulfydryl-containing peptides and proteins oxidize in solution and form disulfide bonds, they must be preventively reduced to free sulfydryls for allowing interaction with maleimide.
96-well microplates, solid or with 12 removable 8-well strips.
A derived maleimide is coated using 100 µl/well. The strips are post-coated (blocked) for low non specific binding and long-term stability.
Microplate was saturated with Glutathione Ethyl Ester Biotin Amide (BioGEE) at a concentration of 2.5 µg/ml (250 ng/well) in an ELISA format using Streptavidin-HRP as detector and TMB as substrate.
The Biomat Maleimide microplate shows a nominal binding capacity of ~ 440 pMol BioGEE/well.
Microplates show a CV% less than 5 when used as a catcher of Glutathione Ethyl Ester Biotin Amide (BioGEE) in an ELISA format using streptavidin-HRP as detector and TMB as substrate.
Storage and Stability
The microplates, if unopened, are stable refrigerated until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.