Maleimide Coated Surface offers a powerful instrument for binding biomolecules containing free sulfhydryl groups or reducible disulfide bonds that are difficult to coat onto polystyrene plates.

Maleimide Coated Surfaces offer a powerful instrument for binding biomolecules containing free sulfhydryl groups (e.g. peptides that contain a terminal cysteine or thiol containing haptens ), or reducible disulfide bonds that are difficult to coat onto polystyrene plates. These coated products are a very useful tool for assays requiring site-directed orientation of particular biomolecules especially during antibody production.

At pH 6.5-7.5 maleimide reacts with free sulfhydryl groups to yield stable bonds, while the reaction with amine becomes significant at pH > 7.5.

If sulfhydryl-containing peptides and proteins oxidize in solution and form disulfide bonds, they must be preventively reduced to free sulfhydryls for allowing interaction with maleimide.

Biomat Maleimide Coated Products

96-Well Plates

Other 96-Well Plates

PCR Products

Other PCR Products

Maleimide Coated Surfaces main features

Biomat Maleimide Coated products have the following characteristics:

  • Ready to Use
  • Manufactured under ISO:9001 guidelines
  • All lots are tested for uniformity and reproducibility
  • Certificate of Quality is released for every lot
  • For Research Use Only

Also, Maleimide Coated Products ensures:

Uniformity

The Maleimide Coated Products  show a CV% less than 10 when used as a catcher of Glutathione Ethyl Ester Biotin Amide (BioGEE) in an ELISA format using streptavidin-HRP as detector and TMB as substrate

Storage and Stability

The Maleimide Coated products, if unopened,  are stable at 2 – 8 °C until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.

Maleimide Coated Microplates Specifications

Coating

A derived maleimide is coated using 100 μl/well.

Binding capacity

Microplate was saturated with Glutathione Ethyl Ester Biotin Amide (BioGEE) at a concentration of 2.5 µg/ml (250 ng/well) in an ELISA format using Streptavidin-HRP as detector and TMB as substrate.

see Binding Capacity technical note

Result:

~ 440 pMol BioGEE/well

Maleimide Coated PCR 8 Strip Tubes and PCR Plates Specifications

Coating

A derived maleimide is coated using 100 μl/tube.