Maleimide coated surfaces offer a powerful instrument for binding biomolecules containing free sulfydryl groups (e.g. peptides that contain a terminal cysteine or thiol containing haptens ) , or reducibile disulfide bonds that are difficult to coat onto polystyrene plates. These coated products are a very useful tool for assays requiring site-directed orientation of particular biomolecules especially during antibody production.
At pH 6.5-7.5 maleimide reacts with free sulfydyl groups to yeld stable bonds, while the reaction with amine becomes significant at pH > 7.5.
If sulfydryl-containing peptides and proteins oxidize in solution and form disulfide bonds, they must be preventively reduced to free sulfydryls for allowing interaction with maleimide.
The coated products show a CV% less than 5 when used as a catcher of Glutathione Ethyl Ester Biotin Amide (BioGEE) in an ELISA format using streptavidin-HRP as detector and TMB as substrate.
Storage and Stability
The coated products, if unopened, are stable at 2-8°C until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.
Product specifications for microplates
A derived maleimide is coated using 100 µl/well. The strips are post-coated (blocked) for low non specific binding and long-term stability.
Microplate was saturated with Glutathione Ethyl Ester Biotin Amide (BioGEE) at a concentration of 2.5 µg/ml (250 ng/well) in an ELISA format using Streptavidin-HRP as detector and TMB as substrate.
The Biomat Maleimide microplate shows a nominal binding capacity of ~ 440 pMol BioGEE/well.
Product specifications for PCR 8 strip tubes and PCR plates
A derived maleimide is coated using using 100 µl/tube. The PCR 8 strip tubes and plates are post-coated (blocked) for low non specific binding and long-term stability.