Maleimide coated surfaces offer a powerful instrument for binding biomolecules containing free sulfydryl groups (e.g. peptides that contain a terminal cysteine or thiol containing haptens ) , or reducibile disulfide bonds that are difficult to coat onto polystyrene plates. These coated products are a very useful tool for assays requiring site-directed orientation of particular biomolecules especially during antibody production.
At pH 6.5-7.5 maleimide reacts with free sulfydyl groups to yeld stable bonds, while the reaction with amine becomes significant at pH > 7.5.
If sulfydryl-containing peptides and proteins oxidize in solution and form disulfide bonds, they must be preventively reduced to free sulfydryls for allowing interaction with maleimide.

Available configurations

Click on the configuration link to scroll down the page to the relative section.

Uniformity

The coated products show a CV% less than 5 when used as a catcher of Glutathione Ethyl Ester Biotin Amide (BioGEE) in an ELISA format using streptavidin-HRP as detector and TMB as substrate.

Storage and Stability

The coated products, if unopened, are stable at 2-8°C until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.

 Product specifications for microplates

Coating

A derived maleimide is coated using 100 µl/well. The strips are post-coated (blocked) for low non specific binding and long-term stability.

Binding capacity

Microplate was saturated with Glutathione Ethyl Ester Biotin Amide (BioGEE) at a concentration of 2.5 µg/ml (250 ng/well) in an ELISA format using Streptavidin-HRP as detector and TMB as substrate.
The Biomat Maleimide microplate shows a nominal binding capacity of ~ 440 pMol BioGEE/well.

see Binding Capacity technical note

Product specifications for PCR 8 strip tubes and PCR plates

Coating

A derived maleimide is coated using using 100 µl/tube. The PCR 8 strip tubes and plates are post-coated (blocked) for low non specific binding and long-term stability.