Protein A Coated Plates are designed for the capture of IgG applied directly or as antibody/antigen complex.
The surface of Protein A Coated Plates provides alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plates-based assay techniques.
Protein A specifically binds the Fc region of immunoglobulins of many mammalian species with an orientation that allows the F(ab)2 binding sites to be freely available for efficient binding to epitope.
Examples of applications for Protein A Coated Plates are:
specific and sterically oriented bond of IgG;
separation of IgG from other immunoglobulins;
separation of antigen-antibodies complexes;
separation of IgG from contaminants;
isolation and analysis of fusion proteins;
finding and identifying red cell antibodies (only on U-bottom plates).
Biomat Protein A Coated 96-Well Plates are made in Transparent, White and Black Polystyrene and can be used for quantitative and qualitative immunoassays like ELISA, Chemiluminescent (CLIA) , Luminescence (LIA) and Fluorescence (FIA) assays.
Protein A Coated Plates are pre-blocked in order to minimize any non-specific binding and to ensure long-term stability.