Wheat Germ Coated Surface offers a powerful and sensitive instrument for binding in specific way the carbohydrate fraction of glycoproteins, enzymes and cell membranes.
Wheat Germ Lectin, belonging to the lectins family, is a Hemagglutinin obtained from the common wheat germ Triticum Vulgaris. It is well known that lectins have been used extensively for the isolation of glyco-conjugates and glycoproteins with specific carbohydrate structures.
Wheat Germ Lectin shows specific affinity for molecules containing N-acetyl-D-glucosamine residue.
Wheat Germ Lectin coated surfaces offer a powerful and sensitive instrument for binding in specific way the carbohydrate fraction of glycoproteins, enzymes and cell membranes.
The optical properties of polystyrene remain unchanged, allowing to use the modified surface as powerful tool for diagnostic assays.
Example of applications:
- studies of surfaces of normal and transformed cells
- glycoprotein purification including membrane glycoproteins
- studies of cell surface changes during development and the cell cycle
Biomat ‘s Wheat Germ Products
Wheat Germ Plates Specifications
Wheat Germ is coated using 200 µl/well. The strips are post-coated (blocked) for low non specific binding and long-term stability.
General procedure for binding a biomolecule
Microplates show a CV% less than 5 when is used anti HIgG-HRP as detector and TMB as substrate.
Storage and Stability
The microplates, if unopened, are stable refrigerated until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.