The Calmodulin (M.W. 1-5 kDa) Ca ++ binding protein is able to bind proteins mainly with hydrophobic sites in its surface.
The polystyrene optical features don’t change, allowing the modified surface to be used as a valid tool to carry out biological tests.
This surface shows its usefulness for these applications:
- interactions with proteins involved in glycogen metabolism
- interactions with factors involved in neurotransmission mechanism
- interactions with enzymes involved in the NAD*/NADP* phosphorilation system
Calmodulin is coated using 200 µl/well. The strips are post-coated (blocked) for low non specific binding and long-term stability.
Microplates show a CV% less than 5 when a monoclonal anti-calmodulin is bound on the surface in an ELISA format, using anti mouse-HRP as detector and TMB as substrate.
Storage and Stability
The microplates, if unopened, are stable refrigerated until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.