Surfaces with carboxylic groups covalently bound are dedicated to promote the covalent immobilisation of compounds containing reactive free amino groups using the EDC mediated amination.
This kind of immobilisation can overcome some of the limitations connected with physical adsorption of the molecules to the surfaces:

  • immobilisation of molecules which are bound weakly or not at all by physical adsorption, namely small peptides (M.W. 1000-5000 Da) drugs, toxins or hormones
  • oriented immobilisation of molecules in order to secure the integrity and accessibility of their specific sites
  • increased storage stability compared with that of physical adsorption because of the reduced risk of spontaneous desorption

Coupling molecules having an amino group to COOH surface

The amino group presents in any molecules, such as peptides or proteins, binds to biomat COOH through formation of amide bonds between the amino group presents in the molecule and the surface carboxylic group by the action of carbodiimide.

see technical note

 

 

Product specifications

Available configurations

96-well microplates, solid or with 12 removable 8-well strips.

Treatment

The surface is modified with carboxylic groups.
Volume of treatment 200 µl/well.

Uniformity

Microplates show a CV% less than 5 when are used as a catcher of biotin-hydrazide in an ELISA format, using streptavidin-HRP as detector and TMB as substrate.

Storage and Stability

The microplates are stored at room temperature in sealed PE bags and are stable until the expiration date printed on the label.