Surfaces with carboxylic groups covalently bound are dedicated to promote the covalent immobilisation of compounds containing reactive free amino groups using the EDC mediated amination.
This kind of immobilisation can overcome some of the limitations connected with physical adsorption of the molecules to the surfaces:
- immobilisation of molecules which are bound weakly or not at all by physical adsorption, namely small peptides (M.W. 1000-5000 Da) drugs, toxins or hormones
- oriented immobilisation of molecules in order to secure the integrity and accessibility of their specific sites
- increased storage stability compared with that of physical adsorption because of the reduced risk of spontaneous desorption
Coupling molecules having an amino group to COOH surface
The amino group presents in any molecules, such as peptides or proteins, binds to biomat COOH through formation of amide bonds between the amino group presents in the molecule and the surface carboxylic group by the action of carbodiimide.
Available configurations
Biomat ‘s Carboxylated surfaces are available on the following Biomat’s products:
Uniformity
The products show a CV% less than 5 when are used as a catcher of biotin-hydrazide in an ELISA format, using streptavidin-HRP as detector and TMB as substrate.
Storage and Stability
The products are stored at room temperature and are stable until the expiration date printed on the label.
Product specifications for microplates
Treatment
The surface is modified with carboxylic groups.
Volume of treatment 200 µl/well.
Carboxylated Microplates Available
Product specifications for PCR 8 strip tubes and PCR plates
Treatment
The surface is modified with carboxylic groups.
Volume of treatment 200 µl/well.
