Protein A specifically binds the Fc region of immunoglobulins of many mammalian species
(see Table 1) with an orientation that allows the F(ab)2 binding sites to be freely available for efficient binding to epitope. When coated on a surfaces, the Protein A can securely capture IgG applied directly or as antigen/antibody complexes.
Example of applications:
- specific and sterically oriented bond of IgG
- separation of IgG from other immunoglobulins
- separation of antigen-antibodies complexes
- separation of IgG from contaminants
- isolation and analysis of fusion proteins
- finding and identifying red cell antibodies (only on U-bottom plates)
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The coated products show a CV% less than 10 when used as a catcher of biotinylated human IgG in an ELISA format using streptavidin-HRP as detector and TMB as substrate.
Storage and Stability
The coated products, if unopened, are stable at 2-8°C until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.
Product specifications for microplates
Recombinant Protein A ( M.W. 38.9 kDa ), from Staphylococcus aureus subsp. Aureus, expressed in E. coli, is coated using 200 µl/well. The strips are post-coated (blocked) for low non specific binding and long-term stability.
Microplate was saturated with biotinylated human IgG at a concentration of 0.4-0.5 µg/ml (400-500 ng/well) in an ELISA format using Streptavidin-HRP diluted mixed with Streptavidin as detector and TMB as substrate
2.66 - 3.33 pMol IgG/well (100 μl volume)
Biotinylated human IgG was detected at a concentration significantly above background in an ELISA format using streptavidin-HRP as detector and TMB as substrate.
0.113 ng/well of human IgG
Product specifications for PCR 8 strip tubes and PCR plates
Recombinant Protein A (M.W. 38.9 kDa), from Staphylococcus aureus subsp. Aureus, expressed in E. coli, is coated using 100 µl/tube. The PCR 8 strip tubes and plates are post-coated (blocked) for low non specific binding and long-term stability.