Protein A specifically binds the Fc region of immunoglobulins of many mammalian species

 (see Table 1)    with an orientation that allows the F(ab)2 binding sites to be freely available for efficient binding to epitope. When coated on a surfaces, the Protein A can securely capture IgG applied directly or as antigen/antibody complexes.

Example of applications:

  • specific and sterically oriented bond of IgG
  • separation of IgG from other immunoglobulins
  • separation of antigen-antibodies complexes
  • separation of IgG from contaminants
  • isolation and analysis of fusion proteins
  • finding and identifying red cell antibodies (only on U-bottom plates)

see Test for finding and identifying red cell antibodies by means of solid phase method

 

Available configurations

Biomat ‘s Protein A/G surfaces are available on the following Biomat’s products:

Uniformity

The coated products show a CV% less than 10 when used as a catcher of biotinylated human IgG in an ELISA format using streptavidin-HRP as detector and TMB as substrate.

Storage and Stability

The coated products, if unopened, are stable at 2-8°C until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.

 Product specifications for microplates

Coating

Recombinant Protein A ( M.W. 38.9 kDa ), from Staphylococcus aureus subsp. Aureus, expressed in E. coli, is coated using 200 µl/well. The strips are post-coated (blocked) for low non specific binding and long-term stability.

Binding capacity

Microplate was saturated with biotinylated human IgG at a concentration of 0.4-0.5 µg/ml (400-500 ng/well) in an ELISA format using Streptavidin-HRP diluted mixed with Streptavidin as detector and TMB as substrate

see Binding Capacity technical note

Results:

2.66 - 3.33 pMol IgG/well (100 μl volume)

Sensitivity

Biotinylated human IgG was detected at a concentration significantly above background in an ELISA format using streptavidin-HRP as detector and TMB as substrate.

see Sensitivity technical note

Results:

0.113 ng/well of human IgG

Product specifications for PCR 8 strip tubes and PCR plates

Coating

Recombinant Protein A (M.W. 38.9 kDa), from Staphylococcus aureus subsp. Aureus, expressed in E. coli, is coated using 100 µl/tube. The PCR 8 strip tubes and plates are post-coated (blocked) for low non specific binding and long-term stability.