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Protein A Coated Surface is designed for the capture of IgG applied directly or as an antibody/antigen complex.
Protein A Coated Surface provides an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plates-based assay techniques.
Protein A specifically binds the Fc region of immunoglobulins of many mammalian species (see Table 1) with an orientation that allows the F(ab)2 binding sites to be freely available for efficient binding to epitope. When coated on a surface, the Protein A can securely capture IgG applied directly or as antigen/antibody complexes.
Examples of applications for Protein A Coated surface
- specific and sterically oriented bond of IgG
- separation of IgG from other immunoglobulins
- separation of antigen-antibodies complexes
- separation of IgG from contaminants
- isolation and analysis of fusion proteins
- finding and identifying red cell antibodies (only on U-bottom plates) (see Test for finding and identifying red cell antibodies by means of solid phase method)
Biomat Protein A Coated Products
Protein A Coated Surfaces main features
Biomat Protein A Coated products have the following characteristics:
- Ready to Use
- Manufactured under ISO:9001 guidelines
- All lots are tested for uniformity and reproducibility
- Certificate of Quality is released for every lot
- For Research Use Only
Also, Protein A Coated Products ensures:
Uniformity
The coated products show a CV% less than 10 when used as a catcher of biotinylated human IgG in an ELISA format using streptavidin-HRP as detector and TMB as substrate.
Storage and Stability
The coated products, if unopened, are stable at 2-8°C until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.
Protein A Coated Microplates Specifications
Coating
Recombinant Protein A ( M.W. 38.9 kDa ), from Staphylococcus aureus subsp. Aureus, expressed in E. coli, is coated using 200 µl/well.
Binding capacity
Microplate was saturated with biotinylated human IgG at a concentration of 0.4-0.5 µg/ml (400-500 ng/well) in an ELISA format using Streptavidin-HRP diluted mixed with Streptavidin as detector and TMB as substrate.
Results:
2.66 - 3.33 pMol IgG/well (100 μl volume)
Sensitivity
Biotinylated human IgG was detected at a concentration significantly above background in an ELISA format using streptavidin-HRP as detector and TMB as substrate.
Results:
0.113 ng/well of human IgG
Protein A Coated PCR 8 Strip Tubes and PCR Plates Specifications
Coating
Recombinant Protein A ( M.W. 38.9 kDa ), from Staphylococcus aureus subsp. Aureus, expressed in E. coli, is coated using 100 µl/tube.
