The Streptavidin Plates are ideal for binding a wide range of biotinylated molecules, making them highly recommended for ELISA and other immunoassays. Streptavidin is one of the most popular biotin-binding proteins in research, with each of its four subunits capable of binding a biotin molecule, resulting in enhanced assay sensitivity and reliability. The streptavidin-coated surface provides a versatile and powerful platform for capturing biotinylated antibodies, antigens, proteins, peptides, polysaccharides, oligonucleotides, DNA fragments, and more. This coating is especially useful for molecules that may not adhere reliably through passive adsorption or that require specific orientation for optimal binding. Using Streptavidin Coated Plates for ELISA ensures improved assay performance, with higher sensitivity and reduced background noise, leading to more accurate and consistent results.
Streptavidin Coated Plates are pre-blocked in order to minimize any non-specific binding and to ensure long-term stability.
Streptavidin Coated 96 Well Plates are available in clear, white, and black polystyrene in Breakable Strip, Strip and Solid format. They are suitable for ELISA, Luminescence, Fluorescence, and Chemiluminescent assays.
- Streptavidin-coated surface: Versatile platform for capturing a wide range of biotinylated molecules, including antibodies, antigens, proteins, peptides, polysaccharides, oligonucleotides, and DNA fragments.Ideal for binding molecules that:Â
- do not adhere reliably through passive adsorption
- require specific orientation for optimal binding.
- Blocking: Plates are pre blocked with standard proteic blocking (ELISA Blocking) or with non proteic blocking (BlockerWell)
- Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
- Wells design: flat bottom.
- Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
- Colors: Available in clear, white, and black. Customizable upper rim colors upon request.
- Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
- Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
- Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
- Alphanumeric Coding: For easy and accurate well identification during experiments.
- Recommended working volume: of 75 to 200 μl
- Packaging: Each coated plate is packed in a single barrier bag with desiccant
- Unit: Contains 5 plates
- Minimum order: 10 plates.
- Ready to use
- Plates Design:Â
- The mould design provides optical quality, important to reduce the background signal.
- The rim protects the external face of the bottom from scratches
- Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
- Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
- Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
- Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.
- ISO 9001 Certified: Produced under strict quality protocols.
- Certificate of Quality: All lots are tested for uniformity and reproducibility and a certificate is released for every lot.
- SBS Standards: Designed for compatibility with automated processing systems, ensuring ease of use in high-throughput labs.
Format Options
Biomat Streptavidin Plates are offered in three formats to accommodate diverse laboratory needs:
- Streptavidin Plates 96-Well Breakable Strip Plates: Composed of 12 breakable strips of 8 wells, these plates allow easy customization by breaking and repositioning strips or single wells as required.Â
- Streptavidin Plates 96-Well Strip Plates: Designed with 12 strips of 8 wells on a rigid frame, preventing misorientation for consistent and accurate assays.
- Streptavidin Plates 96-Well Solid Plates: Solid format designed for high-performance assays.
- Specific and sterically oriented bond of antibodies
- Purification and detection of mouse monoclonal IgG antibodies and for purification of macaque IgG
- Highest specificity and capacity
- Retains antibody activity and orients antibody for maximum binding
- ELISA Assays: For competitive and sandwich ELISA, detecting a wide range of targets such as steroid hormones and antibodies.
- Luminescence Assays (LIA): Optimized for high-sensitivity luminescence detection.
- Fluorescence Assays (FIA): Ensures strong signal detection with minimal interference.
- Chemiluminescent Assays (CLIA): Ideal for highly sensitive and quantitative analyses.
- Other
For further information on the various Biomat 96-Well Plate formats and their specific use cases, contact us.
FOR RESEARCH USE ONLY
The Streptavidin Coated Surface on Streptavidin Coated 96 Well Solid Plates provides a highly effective and versatile platform for binding biotinylated molecules, including proteins, peptides, polysaccharides, oligonucleotides, and DNA fragments. Streptavidin’s strong affinity for biotin allows for robust and stable interactions, making it particularly valuable for applications where passive adsorption is unreliable or where molecules may adsorb in an unfavorable orientation. These plates ensure consistent and oriented binding, which is critical for enhancing assay sensitivity and accuracy in ELISA, nucleic acid capture, and other biochemical assays. Additionally, the high specificity of streptavidin-biotin binding minimizes background noise, offering improved signal clarity in complex assay systems.
High binding streptavidin is a polymer composed of 6–8 streptavidin molecules, offering more biotin-binding sites than standard streptavidin. This results in greater binding capacity for biotinylated molecules. The higher the molecular weight of the biotinylated molecule, the more beneficial the high binding format becomes.
Standard blocking uses BSA (Bovine Serum Albumin), which forms a uniform layer that doesn’t interfere with molecular reactivity. However, rare cross-reactivity with sample components may increase background noise. Non-protein blocking uses inert chemical polymers that eliminate this risk, making them ideal for sensitive assays or when unknown sample interference is a concern.
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Streptavidin-coated 96 well plates provide high affinity for biotinylated molecules, making them ideal for ELISA formats involving biotin-tagged antibodies or antigens. This specific binding improves both sensitivity and specificity, ensuring reliable detection and quantification of target analytes.
Yes, streptavidin-coated plates are highly versatile and suitable for applications such as protein-protein interaction studies, immunoassays, and molecular binding assays. Their ability to immobilize biotinylated targets makes them valuable in a wide range of biochemical and diagnostic experiments.
To maintain coating stability, streptavidin plates should be stored at 2–8°C, protected from moisture and light, and kept in their original sealed packaging. Proper storage conditions ensure functional integrity and consistent assay results.
Depending on the formulation, our streptavidin-coated 96 well plates have a shelf life of 24 to 36 months. Adhering to the recommended storage conditions ensures that performance and binding efficiency remain stable throughout the shelf life.
Yes, when transitioning from another plate type, protocols may need to be adjusted—particularly regarding the concentration and purity of biotinylated reagents. Because streptavidin binds biotin very strongly, optimizing reagent titration is critical to avoid oversaturation. Our technical team is available to support protocol refinement and integration.
